Surface immobilization of bioactive molecules on polyurethane for promotion of cytocompatibility to human endothelial cells
Surface immobilization of bioactive molecules on polyurethane for promotion of cytocompatibility to human endothelial cells
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DOI：
10.1002/mabi.200390020
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发表时间：
2003-04-14
影响因子：
4.6
通讯作者：
Shen, JC
中科院分区：
文献类型：
--
作者：
Gao, CY;Guan, JJ;Shen, JC
细胞相容性聚合物在组织工程支架和植入装置中越来越重要。采用紫外光引发甲基丙烯酸（MAA）接枝聚合改性聚氨酯（PU）膜，以1-乙基-3-（3-二甲氨基丙基）盐酸碳二亚胺为凝聚剂，对明胶或精氨酸-甘氨酸-天冬氨酸（RGD）肽进行共价固定。通过FTIR衰减全反射（ATR）光谱和x射线光电子能谱（XPS）证实了明胶或RGD肽的固定化。体外培养内皮细胞的结果表明，明胶或rGD肽修饰的PU膜比对照PU或PMAA接枝的PU膜具有更好的细胞相容性。[图]pu -接枝- pmaa (a)、pu -接枝- pmaa -接枝- rgd (b)的ATR-FTIR光谱及其相减结果(c)。PMAA接枝度为21.56 × 10(-2) mg/cm(2)。
Cytocompatible polymers are of increasing importance for tissue engineering scaffolds and implanted devices. Polyurethane (PU) membranes were modified by grafting polymerization of methacrylic acid (MAA) initiated by UV light and further covalent immobilization of gelatin or arginine-glycine-aspartic (RGD) peptide using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride as a condensing agent. The immobilization of gelatin or RGD peptide was confirmed by FTIR attenuated total reflection (ATR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The results for endothelial cells cultured in vitro proved that the PU membrane modified with gelatin or rGD peptide had better cytocompatibility than the control PU or the PMAA grafted PU membrane.[GRAPHICS]ATR-FTIR spectra of PU-graft-PMAA (a), PU-graft-PMAA-graft-RGD (b), and their subtraction result (c). The PMAA grafting degree is 21.56 x 10(-2) mg/cm(2).