Cocaine and benzoylecgonine in saliva, serum, and urine.
Cocaine and benzoylecgonine in saliva, serum, and urine.
复制标题
DOI：
10.1093/clinchem/39.3.481
复制
发表时间：
1993-03
影响因子：
9.3
通讯作者：
W. Schramm;P. Craig;R. Smith;G. E. Berger
中科院分区：
文献类型：
--
作者：
W. Schramm;P. Craig;R. Smith;G. E. Berger
我们研究了使用唾液检测可卡因和苯甲酰爱康宁的可行性。使用渗透装置将唾液作为超滤液直接收集在口腔中。我们通过免疫分析匹配的尿液、血液和唾液超滤液样本进行了分析，这些样本来自 69 名在样本采集后 24 小时内使用过可卡因的患者。唾液中的可卡因浓度是血清中的 4.9 倍；血清中苯甲酰爱康宁浓度高出2.5倍。 7 份尿液样本和 2 份血清样本中可卡因浓度无法检测到，但所有 69 份唾液样本均呈药物阳性。对于苯甲酰爱康宁检测，所有尿液样本均呈阳性，三份血清样本和一份唾液样本呈阴性。我们还通过气相色谱/质谱分析了 43 个唾液样本：所有样本均呈苯甲酰爱康宁阳性，而 40 个样本呈可卡因阳性。我们的结论是，同时测量唾液中的可卡因和苯甲酰爱康宁适用于筛查近期可卡因使用情况。
We studied the feasibility of using saliva to detect cocaine and benzoylecgonine. Saliva was collected as an ultrafiltrate directly in the mouth with an osmotic device. We analyzed by immunoassay matched samples of urine, blood, and salivary ultrafiltrate from 69 patients who had used cocaine within 24 h of sample collection. Cocaine concentrations were 4.9 times higher in saliva than in serum; benzoylecgonine concentrations were 2.5 times higher in serum. Seven urine and two serum samples had undetectable concentrations of cocaine, but all 69 saliva samples were positive for the drug. For benzoylecgonine detection, all urine samples were positive and three serum and one saliva sample were negative. We also analyzed 43 samples of saliva by gas chromatography/mass spectrometry: all were positive for benzoylecgonine, whereas 40 were positive for cocaine. We conclude that simultaneous measurement of cocaine and benzoylecgonine in saliva is suitable in screening for recent cocaine use.