Polycaprolactone/carboxymethyl chitosan nanofibrous tissue engineering application
Polycaprolactone/carboxymethyl chitosan nanofibrous tissue engineering application
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DOI：
10.1016/j.ijbiomac.2018.04.045
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发表时间：
2018-08-01
影响因子：
8.2
通讯作者：
Bakhshi, Hadi
中科院分区：
文献类型：
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作者：
Sharifi, Fereshteh;Atyabi, Seyed Mohammad;Bakhshi, Hadi
本研究主要研究了聚己内酯/壳聚糖(PCL/CTS)和PCL/羧甲基壳聚糖(PCL/CMC)纳米纤维支架材料在骨组织工程中的物理性能和细胞相容性。支架材料的制备采用静电纺丝技术。扫描电子显微镜照片表明，用CMC取代CTS可以消除PCL/CTS支架中不需要的超细纤维和裂解纤维。与PCL/CTS支架相比，PCL/CMC支架的表面亲水性显著提高。在高浓度CMC作用下，加入15%CMC后，PCL支架的水接触角由123+/-1度降至51+/-3度。在PCL/CTS 15%和PCL/CMC 15%中，纤维的平均直径分别为439和356 nm，说明CMC的浓度越高，纤维的直径越小。FTIR分析证实了PCL/CTS和PCL/CMC支架的组成。人成骨细胞(MG63)在支架上的培养表明，所有支架均具有良好的生物相容性。与PCL和PCL/CTS纳米纤维相比，PCL/CMC纳米纤维表现出促进增殖的趋势，尤其是在CMC的最大浓度下。结果表明，PCL/CMC电纺支架具有良好的骨组织工程应用前景。(C)2018年，由爱思唯尔出版。
This research focused on the physical properties and cell compatibility of nanofibrous scaffolds based on polycaprolactone/chitosan (PCL/CTS) and PCL/carboxymethyl chitosan (PCL/CMC) blends for bone tissue engineering application. Scaffolds were fabricated by electrospinning technique. SEM images showed that the undesirable ultrafine and splitting fibers in PCL/CTS scaffolds are eliminated by replacing CTS with CMC. PCL/CMC scaffolds exposed significantly improved surface hydrophilicity improvement comparing to PCL/CTS ones. The water contact angle of PCL scaffold was reduced on the addition of 15% CMC from 123 +/- 1 degrees to 51 +/- 3 degrees in high concentration of CMC scaffold. The average diameter of fibers in PCL/CTS 15% and PCL/CMC 15% were 439 and 356 nm, respectively, which demonstrated higher concentrations of CMC resulted in decrease fibers diameter than other blended scaffolds. FTIR spectroscopy confirmed the composition of PCL/CTS and PCL/CMC scaffolds. The culturing of human osteoblast cells (MG63) on the scaffolds showed that all scaffolds are biocompatible. The PCL/CMC nanofibers exhibited promoting proliferation trend, compared to the PCL and PCL/CTS ones, especially at maximum concentrations of CMC. The results demonstrate that the PCL/CMC electrospun scaffolds can be an excellent candidate for bone tissue engineering application. (C) 2018 Published by Elsevier B.V.