Mismatch repair-driven mutational bias in D. melanogaster
Mismatch repair-driven mutational bias in D. melanogaster
复制标题
DOI：
10.1016/s1097-2765(02)00575-0
复制
发表时间：
2002-07-01
期刊：
影响因子：
16
通讯作者：
Schlötterer, C
中科院分区：
文献类型：
--
作者：
Harr, B;Todorova, J;Schlötterer, C
我们利用微卫星序列来评估错配修复系统对D.黑腹果蝇虽然错配熟练的细胞在（GT）（n）重复处具有最高的突变率，但在缺乏功能性错配修复的spe/1（-）/（-）果蝇中，（AT）重复是最不稳定的重复。此外，spel 1（-）/（-）中长微卫星等位基因的突变谱略微向上偏置，导致重复序列的增加，而野生型果蝇具有强烈的向下偏置。有趣的是，这种错配修复介导的向下突变偏好反映在D.黑腹菌与其他物种相比，D.黑腹果蝇的微卫星明显较短。我们的研究结果表明，错配修复系统可能有一个重要的作用，在塑造基因组组成。
We have used microsatellite sequences to evaluate the influence of the mismatch repair system on mutation bias in D. melanogaster. While mismatch-proficient cells have the highest mutation rate at (GT)(n) repeats, (AT), repeats were the least stable ones in spe/1(-)/(-) flies lacking functional mismatch repair. Furthermore, the mutation spectrum of long microsatellite alleles in spel1(-)/(-) was slightly upward biased, resulting in a gain of repeats, whereas wild-type flies have a strong downward bias. Interestingly, this mismatch repair-mediated downward mutation bias is reflected in the genome composition of D. melanogaster. When compared to other species, D. melanogaster has significantly shorter microsatellites. Our results suggest that the mismatch repair system may have an important role in shaping genome composition.