Control of genic DNA methylation by a jmjC domain -: Containing protein in Arabidopsis thaliana
Control of genic DNA methylation by a jmjC domain -: Containing protein in Arabidopsis thaliana
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DOI：
10.1126/science.1150987
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发表时间：
2008-01-25
期刊：
影响因子：
56.9
通讯作者：
Kakutani, Tetsuji
中科院分区：
文献类型：
--
作者：
Saze, Hidetoshi;Shiraishi, Akiko;Kakutani, Tetsuji
重复序列和基因的差异胞嘧啶甲基化对于基因组稳定性和基因表达的协调是重要的。通过在基因区域显示异位胞嘧啶甲基化的突变体的遗传筛选，我们在拟南芥中发现了一个jmjC-结构域基因IBM1（增加盆景甲基化1）。除了异位胞嘧啶甲基化外，ibm1突变还诱导了多种发育表型，这些表型依赖于赖氨酸9处组蛋白H3的甲基化。矛盾的是，ibm1的发育表型通过染色质重塑基因DDM1的突变（DNA甲基化1的减少）而增强，这对于保持重复异染色质位点的甲基化和沉默是必要的。我们的研究结果证明了染色质重塑和组蛋白修饰在重复序列和基因的差异表观遗传控制中的重要性。
Differential cytosine methylation of repeats and genes is important for coordination of genome stability and proper gene expression. Through genetic screen of mutants showing ectopic cytosine methylation in a genic region, we identified a jmjC- domain gene, IBM1 ( increase in bonsai methylation 1), in Arabidopsis thaliana. In addition to the ectopic cytosine methylation, the ibm1 mutations induced a variety of developmental phenotypes, which depend on methylation of histone H3 at lysine 9. Paradoxically, the developmental phenotypes of the ibm1 were enhanced by the mutation in the chromatin- remodeling gene DDM1 ( decrease in DNA methylation 1), which is necessary for keeping methylation and silencing of repeated heterochromatin loci. Our results demonstrate the importance of chromatin remodeling and histone modifications in the differential epigenetic control of repeats and genes.