STABILIZATION OF BROME MOSAIC-VIRUS
STABILIZATION OF BROME MOSAIC-VIRUS
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DOI：
10.1098/rstb.1976.0100
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发表时间：
1976-01-01
影响因子：
6.3
通讯作者：
HIRTH, L
中科院分区：
文献类型：
--
作者：
PFEIFFER, P;HERZOG, M;HIRTH, L
根据以下实验证据，讨论了蛋白质-蛋白质和RNA-蛋白质相互作用在稳定雀麦花叶病毒颗粒结构中的相对重要性：病毒颗粒的分解途径、病毒的重组和蛋白质部分的自结合能力、二价阳离子在病毒稳定中的作用及其与病毒颗粒中RNA定位的相关性。BMV的衣壳在低pH时可能主要由疏水键稳定，但在中性附近和中性以上则不稳定，因为RNA-蛋白质的静电相互作用对病毒颗粒的完整性是必不可少的。提出了BMV在不同构型状态下的结构模型。
The relative importances of protein-protein and RNA-protein interactions in stabilizing the architecture of brome mosaic virus particles are discussed in the light of the following experimental evidence: disassembly pathways of the virus particles, reassembly of the virus and self-association capacity of the protein moiety, and the role of divalent cations in virus stabilization and their relevance to localization of the RNA in the virus particles. The capsid of BMV may be primarily stabilized by hydrophobic bonds at low pH, but not around and above neutrality where RNA-protein electrostatic interactions are essential to the integrity of the virus particles. A model is proposed for the structure of BMV in the different configurational states.