Purification, crystallization and preliminary X-ray analysis of a Nup107-Nup133 heterodimeric nucleoporin complex.
Purification, crystallization and preliminary X-ray analysis of a Nup107-Nup133 heterodimeric nucleoporin complex.
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Nup107-Nup133 异二聚核孔蛋白复合物的纯化、结晶和初步 X 射线分析。
DOI：
10.1107/s1744309107040523
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Schwartz,ThomasU
中科院分区：
文献类型：
--
作者：
Boehmer,Thomas;Schwartz,ThomasU
核孔复合体(NPC)是胞核和细胞质之间运输的唯一通道，由大约30种蛋白质的多个拷贝组成，统称为核孔蛋白(Nups)。NUP被组织成不同的亚复合体。Nup107和Nup133是基本Nup107-160亚复合体的成员，Nup107-160亚复合体是中央NPC架构的组成部分。用双顺反子载体在大肠杆菌中表达了人Nup107和Nup133 C-端结构域的二聚体，纯化并结晶成两种不同的晶型。在18-22%PEG3350存在下生长的晶体属于P212121空间群，衍射率为2.9 ä。在1.1 M丙二酸钠存在下生长的天然晶体和含硒L蛋氨酸衍生物晶体属于C2空间群，其衍射峰分别为2.5 5和2.9 ä。对这种复合体的结构确定将使人们首次深入了解NPC核心模块中的蛋白质-蛋白质相互作用。
The nuclear pore complex (NPC), the sole gateway of traffic between the nucleus and the cytoplasm, is built up from multiple copies of about 30 proteins collectively termed nucleoporins (nups). Nups are organized into distinct subcomplexes. Nup107 and Nup133 are members of the essential Nup107–160 subcomplex, a component of the central NPC architecture. A dimeric complex of the C-terminal domains of human Nup107 and Nup133 was expressed from a bicistronic vector in Escherichia coli, purified and crystallized in two different crystal forms. Crystals grown in the presence of 18–22% PEG 3350 belong to space group P212121 and diffracted to 2.9 Å. Native and seleno-l-methionine-derivative crystals grown in the presence of 1.1 M sodium malonate belong to space group C2 and diffracted to 2.55 and 2.9 Å, respectively. Structure determination of this complex will give the first insights into the protein–protein interactions within a core module of the NPC.
影响因子：
3.3
作者：
Orjalo, Arturo V.;Arnaoutov, Alexei;Forbes, Douglass J.
通讯作者：
Forbes, Douglass J.
影响因子：
16
作者：
Harel, A;Orjalo, AV;Forbes, DJ
通讯作者：
Forbes, DJ