Genetic basis of the historical iron-accumulating dgl and brz mutants in pea
Genetic basis of the historical iron-accumulating dgl and brz mutants in pea
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豌豆历史铁积累dgl和brz突变体的遗传基础
DOI：
10.1111/tpj.16514
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Harrington S
中科院分区：
文献类型：
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作者：
Harrington S
豌豆变异体退化叶(DGL)和青铜变异体(BRZ)在叶中积累大量铁。这两个突变体在几十年前首次被描述，为植物中铁的动态平衡提供了重要的见解，但潜在的突变仍然未知。利用外显子组测序，我们确定了与dglin aBRUTU Shomolog相关的框内缺失。野生型和原始亲本中不存在该缺失。Brutus属于E3泛素连接酶的一个小家族，它是植物铁吸收的负调节因子。这个突变之前被定位在第4号染色体上，将这个区域叠加到豌豆基因组序列上，发现了OPT3的一个突变，它编码了一种寡肽转运蛋白，在金属运输中具有植物特有的作用。这些突变的因果性质通过额外的遗传分析得到了证实。突变基因的鉴定使之前描述的许多表型合理化，并为缺铁的地上部到根信号提供了新的见解。此外，这些基本基因中的非致命性突变表明了用铁生物强化作物的新策略。
ThePisum sativum(pea) mutantsdegenerate leaves(dgl) andbronze(brz) accumulate large amounts of iron in leaves. First described several decades ago, the two mutants have provided important insights into iron homeostasis in plants but the underlying mutations have remained unknown. Using exome sequencing we identified an in‐frame deletion associated withdglin aBRUTUShomolog. The deletion is absent from wild type and the original parent line. BRUTUS belongs to a small family of E3 ubiquitin ligases acting as negative regulators of iron uptake in plants. Thebrzmutation was previously mapped to chromosome 4, and superimposing this region to the pea genome sequence uncovered a mutation inOPT3, encoding an oligopeptide transporter with a plant‐specific role in metal transport. The causal nature of the mutations was confirmed by additional genetic analyses. Identification of the mutated genes rationalizes many of the previously described phenotypes and provides new insights into shoot‐to‐root signaling of iron deficiency. Furthermore, the non‐lethal mutations in these essential genes suggest new strategies for biofortification of crops with iron.