Fracture healing and bone mass in rats fed on liquid diet containing ethanol
Fracture healing and bone mass in rats fed on liquid diet containing ethanol
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DOI：
10.1111/j.1530-0277.2002.tb02568.x
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发表时间：
2002-04-01
影响因子：
3.2
通讯作者：
Bora, A
中科院分区：
文献类型：
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作者：
Elmali, N;Ertem, K;Bora, A
背景资料：在酒精滥用的动物模型的研究表明，乙醇抑制骨生长，减少骨形成，并增加骨折risk.Methods：实验胫骨骨折愈合大鼠喂含7.2%乙醇的液体饮食8周进行了研究，组织学和骨密度研究与对照组。经过4周的维生素A和富含蔗糖的牛奶含有7.2%的乙醇喂养，我们创造了闭合性胫骨骨折，然后用髓内钉固定，在10只大鼠。随访4周后，处死大鼠进行检查。另取10只大鼠作为对照组，给予相同的饲料（等热量改良流质饲料），但不含乙醇。骨折愈合的组织学评分系统的开发为fracture health.Results：骨折区域的组织学评价显示，平均骨折愈合评分为1.9在乙醇喂养组与2.6在对照组（p = 0.014）。在试验组中，骨折区域的双能X线吸收测量显示平均骨密度为0.11 +/- 0.03 g/cm（2），而对照组为0.130 +/- 0.051 g/cm（2）（p = 0.000）。试验组骨折区域的平均骨矿物质含量为0.103 +/- 0.08 g/cm（3），对照组为0.128 +/- 0.06 g/cm（3）（p = 0.000）。组织学评分、骨密度（r = 0.64，p = 0.04）和骨矿物质含量（r = 0，63，p = 0.04）之间存在显著相关性。除了酒精对骨代谢的负面影响外，它还干扰骨愈合过程。
Background: Studies in animal models for alcohol abuse have suggested that ethanol inhibits bone growth, decreases bone formation, and increases fracture risk.Methods: Experimental tibia fracture healing in rats fed a liquid diet containing 7.2% ethanol for 8 weeks was investigated with histological and osteodensitometric studies with respect to the control group. After 4 weeks of vitamin A- and sucrose-enriched milk containing 7.2% ethanol feeding, we created closed tibia fractures, which were then fixed with intramedullary nails, in 10 rats. After a follow-up time of 4 weeks, the rats were killed for examination. The same procedure was performed in another 10 rats, which were fed on the same diet (isocaloric modified liquid diet) but without ethanol and used as the control group. A histological scoring system was developed for fracture healing.Results: Histological evaluation of fracture region revealed an average fracture healing score of 1.9 in the ethanol-fed group versus 2.6 in the control group (p = 0.014). In the test group, dual-energy x-ray absorptiometry measurements in the fracture region showed a mean bone mineral density of 0.11 +/- 0.03 g/cm(2), whereas it was 0.130 +/- 0.051 g/cm(2) in the control group (p = 0.000). The mean bone mineral content in the fracture region was 0.103 +/- 0.08 g/cm(3) in the test group versus 0.128 +/- 0.06 g/cm(3) in the control group (p = 0.000). A significant correlation was found among histological scores, bone mineral density (r = 0.64, p = 0.04), and bone mineral content (r = 0,63, p = 0.04).Conclusions: This study showed that rats fed on a diet mixed with ethanol have a histologically delayed fracture healing associated with decreased bone density and mineral content. Besides the negative effects of ethanol on bone metabolism, it also interferes with the fracture-healing process.