Phylogenetic comparison of Leucocytozoon spp. from wild birds of Japan
Phylogenetic comparison of Leucocytozoon spp. from wild birds of Japan
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DOI：
10.1292/jvms.69.55
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发表时间：
2007-01-01
影响因子：
1.2
通讯作者：
Murata, Koichi
中科院分区：
文献类型：
--
作者：
Sato, Yukita;Hagihara, Mio;Murata, Koichi
利用显微镜分析发现8种日本鸟类感染了白细胞虫属。我们利用PCR和线粒体细胞色素B基因（cyt B）的序列分析来比较这些原生动物物种之间的遗传背景。8种鸟类中有6种在22个样本的20个个体中检测到单一扩增带，其中9种为松鸡（Lagopus mutus），4种为大嘴乌鸦（Corvus macrorhynchos），2种为食腐乌鸦（C. corone）、角鸮（Otus scops）2只、日本蜡嘴雀（Eophona personata）1只和棕耳石首鱼（Hypsipetes amaurotis）2只。基于部分cyt B序列的系统发育分析显示，日本的所有Leucocytozoon分离株与文献中报道的其他Leucocytozoon种紧密分组。系统发育树显示日本分离株中L.来自日本岩雷鸟的lovati可能是在其他鸟类中发现的寄生虫的基础。我们的研究是第一个确定日本鸟类区系中白细胞虫寄生虫之间的分子关系。
Eight species of Japanese birds were found to be infected with Leucocytozoon species using microscopic analysis. We used PCR and sequence analysis of the mitochondrial cytochrome b gene (cyt b) to compare the genetic background among these detected protozoa species. In 20 individuals of 22 samples, a single amplified band was detected from 6 of 8 bird species; 9 Japanese rock ptarmigans (Lagopus mutus japonicus), 4 large-billed crows (Corvus macrorhynchos), 2 carrion crows (C. corone), 2 scops owls (Otus scops), I Japanese grosbeak (Eophona personata), and 2 brown-eared bulbuls (Hypsipetes amaurotis), respectively. Phylogenetic analysis based on the partial cyt b sequences revealed that all Leucocytozoon isolates in Japan closely grouped with other Leucocytozoon species previously reported in the literature. Among the Japanese isolates, the phylogenetic tree suggested that L. lovati from the Japanese rock ptarmigan may be basal to the parasites found in other bird species. Our study is the first to identify the molecular relationships among Leucocytozoon parasites in the avifauna of Japan.