2,6-dichlorobenzonitrile, a cellulose biosynthesis inhibitor, affects morphology and structural integrity of petunia and lily pollen tubes
2,6-dichlorobenzonitrile, a cellulose biosynthesis inhibitor, affects morphology and structural integrity of petunia and lily pollen tubes
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DOI：
10.1078/0176-1617-00651
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发表时间：
2002-01-01
影响因子：
4.3
通讯作者：
Bedinger, P
中科院分区：
文献类型：
--
作者：
Anderson, JR;Barnes, WS;Bedinger, P
关于纤维素在花粉管壁上的存在及其功能一直存在争议。本研究通过纤维素生物合成特异性抑制剂2,6-二氯苯腈（DCB）体外培养花粉管，分析了纤维素在花粉管生长中的作用。在0.25 ~ 10 μ mol/L DCB的条件下，对金莲和矮牵牛的花粉管进行体外培养，虽然不影响花粉萌发和生长速度，但矮牵牛和百合的花粉管在DCB的作用下经常破裂或呈现扭曲的球茎形状，当在正常生长的矮牵牛花粉管中加入DCB后，花粉管在15 min内破裂。免疫定位研究表明，经DCB处理的百合花粉管发生胼胝质沉积。DCB处理的百合花粉管超微结构检查显示花粉管壁高度紊乱。我们的研究结果表明，在DCB的存在下，纤维素的减少或缺乏会导致花粉管细胞壁的明显扭曲和减弱。
There has beer an ongoing debate about the presence and function of cellulose in pollen tube walls. In this study, the role of cellulose in pollen tube growth was analyzed by growing pollen tubes in vitro in the presence of 2,6-dichlorobenzonitrile (DCB), a specific inhibitor of cellulose biosynthesis. Pollen tubes of Lilium auratum and Petunia hybrida were cultured In vitro in the presence of 0.25 to 10 mumol/L DCB, Although pollen germination and growth rate were not affected, petunia and lily pollen tubes frequently ruptured or assumed a distorted, bulbous shape in the presence of DCB, When DCB was added to normally growing petunia pollen tubes, pollen tube rupture was observed within 15 min. Immunolocalization studies showed that in lily pollen tubes treated with DCB, callose deposition occurred. Ultrastructural examination of DCB treated lily pollen tubes revealed a highly disorganized pollen tube wall. Our results suggest that the reduction or lack of cellulose in the presence of DCB causes significant distortion and weakening of the pollen tube cell wall.