IMMUNOHISTOCHEMICAL AND ELECTROCHEMICAL DETECTION OF SEROTONIN IN THE NERVOUS-SYSTEM OF THE BLOOD-FEEDING BUG, RHODNIUS-PROLIXUS
IMMUNOHISTOCHEMICAL AND ELECTROCHEMICAL DETECTION OF SEROTONIN IN THE NERVOUS-SYSTEM OF THE BLOOD-FEEDING BUG, RHODNIUS-PROLIXUS
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DOI：
10.1002/arch.940080305
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发表时间：
1988-01-01
影响因子：
2.2
通讯作者：
LLOYD, RJ
中科院分区：
文献类型：
--
作者：
LANGE, AB;ORCHARD, I;LLOYD, RJ
5-羟色胺的分布在整个神经系统的吸血虫Rhodnius prolixus已研究使用免疫组织化学和反相高效液相色谱电化学检测耦合。大约有150个类胡萝卜素免疫反应神经元分布在整个中枢神经系统。这些神经元分布在腹侧和背侧表面上，并且在所有神经节中被发现。这些神经元中的几个似乎是同源的，以前描述的昆虫中的类胡萝卜素免疫反应神经元。这些包括一对双侧对称的神经元，其轴突从食管下神经节伸出，以及一些连续同源的神经元，其向对侧伸出，似乎是中间神经元。免疫反应性分支和静脉曲张被发现在所有的神经节的神经堆，和免疫反应性轴突被发现在所有的神经节间的连接。一些周围神经被免疫反应性突起丛所覆盖。这些过程导致了一个细小的静脉曲张分支的网状结构，位于周围神经的表面，类似于神经血管区。Rhodnius的中枢神经系统含有约5.5 pmol的5-羟色胺，在神经节之间不均匀分布。脑和视叶中含量最高，胸前神经节中含量最低。大量的5-羟色胺存在于心脏体和具有类胡萝卜素神经血管区的周围神经中。血清素是从这些神经血管区释放的，以钙依赖的方式响应高钾生理盐水。5-羟色胺在该虫体内起着重要的中枢和外周作用。
The distribution of serotonin throughout the nervous system of the blood-feeding bug Rhodnius prolixus has been studied using immunohistochemistry and reversed-phase high-performance liquid chromatography coupled to electrochemical detection. Approximately 150 serotonin-like immunoreactive neurons are distributed throughout the central nervous system. These neurons are distributed over both ventral and dorsal surfaces and are found in all ganglia. Several of these neurons appear to be homologous to previously described serotonin-like immunoreactive neurons in insects. These include a large pair of bilaterally symmetrical neurons that project axons out of the suboesophageal ganglion, and some serially homologous neurons which project contralaterally and appear to be interneurons. Immunoreactive branches and varicosities are found in the neuropile of all ganglia, and immunoreactive axons are found in all interganglionic connectives. Several of the peripheral nerves are covered in a plexus of immunoreactive processes. These processes result in a meshwork of fine varicose branches lying superficially over the peripheral nerves, which resemble neurohemal areas. The central nervous system of Rhodnius contains about 5.5 pmol serotonin unequally distributed between the ganglia. The highest content is found in the brain and optic lobes, and the lowest content is found in the prothoracic ganglion. Substantial amounts of serotonin are present in the corpus cardiacum and in the peripheral nerves possessing the serotonin-like neurohemal areas. Serotonin is released from these latter neurohemal areas in a calcium-dependent manner in response to high-potassium saline. It is concluded that serotonin plays an important central and peripheral role in this insect.