Spiral tube support for high-speed countercurrent chromatography
Spiral tube support for high-speed countercurrent chromatography
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DOI：
10.1080/10826070802019913
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发表时间：
2008-01-01
影响因子：
1.3
通讯作者：
Finn, T. M.
中科院分区：
文献类型：
--
作者：
Ito, Y.;Clary, R.;Finn, T. M.
介绍了一种新型的螺旋管支架，其允许容纳由长段氟化塑料管制成的多层螺旋柱。本研究中测试的两个螺旋柱由1.6mm ID和0.85mm ID的FEP（氟化乙烯丙烯）和PTFE（聚四氟乙烯）管组成，总容量分别为100 mL和40 mL。在体积比为4：1：5的正丁醇/乙酸/水的两相溶剂体系中，以正丙基酪氨酸（try-tyr）和缬氨酰酪氨酸（val-tyr）为供试品，在不同转速和流速下考察了这些螺旋柱的性能。在所测试的4种不同洗脱模式中，L-I-T（从螺旋柱的内部头端泵送的下相）和U-O-H（从螺旋柱的外部尾部泵送的上相）在低流速下产生最好的结果，特别是对于两个柱。当流速为1 mL/min时，U-O-H色谱峰的分离度（Rs）大于3.5，固定相保留率为74%。在5 mL/min的流速下，1，200 rpm的最高转速提高了两种洗脱模式下的峰分离度。本系统可用于生物医学研究中各种极性化合物的纯化。
A novel spiral tube support is introduced which allows accommodating a multlilayer spiral column made of a long piece of fluorinated plastic tubing. Two spiral columns tested in this study consist of 1.6mm ID and 0.85mm ID FEP (fluorinated ethylenepropylene) and PTFE (polytetrafluoroethylene) tubing with the total capacity of 100mL and 40mL, respectively. Performance of these spiral columns was examined with a two phase solvent system composed of 1-butanol/acetic acid/water at a volume ratio of 4:1:5 using tryptophyl-tyrosine (try-tyr) and valyl-tyrosine (val-tyr) as test samples under various revolution speeds and flow rates. Among 4 different elution modes tested, L-I-T (lower phase pumped from the internal head end of the spiral column) and U-O-H (upper phase pumped from the external tail of the spiral column) at a low flow rate produced the best results especially for both columns. The highest peak resolution (Rs) of over 3.5 was obtained from U-O-H at a flow rate of 1mL/min with 74% stationary phase retention. At a flow rate of 5mL/min, the highest revolution speed at 1,200rpm improved the peak resolution in both elution modes. The present system may be useful for purification of various polar compounds in biomedical researches.