RIBOSOMAL FRAMESHIFTING EFFICIENCY AND GAG GAG-POL RATIO ARE CRITICAL FOR YEAST M(1) DOUBLE-STRANDED-RNA VIRUS PROPAGATION
RIBOSOMAL FRAMESHIFTING EFFICIENCY AND GAG GAG-POL RATIO ARE CRITICAL FOR YEAST M(1) DOUBLE-STRANDED-RNA VIRUS PROPAGATION
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DOI：
10.1128/jvi.66.6.3669-3676.1992
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发表时间：
1992-06-01
影响因子：
5.4
通讯作者：
WICKNER, RB
中科院分区：
文献类型：
--
作者：
DINMAN, JD;WICKNER, RB
翻译酵母L-A双链RNA病毒正链的gag开放阅读框的核糖体的约1.9%经历α-1移码并在pol开放阅读框中继续翻译以产生170-kDa的gag-pol融合蛋白。移码效率对病毒繁殖的重要性在一个系统中进行了测试，其中M1（杀伤毒素编码）卫星RNA由全长L-A cDNA克隆支持。无论是增加或减少移码效率超过两倍的改变，在光滑的网站中断病毒的繁殖。由染色体突变hsh 1（高移）引起的三倍增加也有同样的效果。以正确的效率取代Tyl的+1核糖体移码位点也允许支持Mi繁殖。正常的-1移码效率与病毒颗粒中观察到的170-kDa gag-pol蛋白与70-kDa gag基因产物（主要外壳蛋白）的摩尔比相似。结果解释的包装模型的L-A。
About 1.9% of ribosomes translating the gag open reading frame of the yeast L-A double-stranded RNA virus positive strand undergo a - 1 frameshift and continue translating in the pol open reading frame to make a 170-kDa gag-pol fusion protein. The importance of frameshifting efficiency for viral propagation was tested in a system where the M1 (killer toxin-encoding) satellite RNA is supported by a full-length L-A cDNA clone. Either increasing or decreasing the frameshift efficiency more than twofold by alterations in the slippery site disrupted viral propagation. A threefold increase caused by a chromosomal mutation, hsh1 (high shifter), had the same effect. Substituting a +1 ribosomal frameshift site from Tyl with the correct efficiency also allowed support of M, propagation. The normal -1 frameshift efficiency is similar to the observed molar ratio in viral particles of the 170-kDa gag-pol protein to the 70-kDa gag gene product, the major coat protein. The results are interpreted in terms of a packaging model for L-A.