Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography
Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography
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DOI：
10.1007/s10753-015-0119-z
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发表时间：
2015-02
期刊：
影响因子：
5.1
通讯作者：
Yuanyuan Wang;Jing Zhao;Lifei Tan;Yueyue Huang;Dequan Li;Shichao Quan;Min Li;Jingye Pan
中科院分区：
文献类型：
--
作者：
Yuanyuan Wang;Jing Zhao;Lifei Tan;Yueyue Huang;Dequan Li;Shichao Quan;Min Li;Jingye Pan
内皮细胞功能障碍在脓毒症的发生和发展中起重要作用，是凝血和炎症相互作用的结果。犬尿氨酸（KYN）是一种内皮源性舒张因子，对脓毒症的病理生理学有很大贡献。在这项研究中，我们研究了骨髓间充质干细胞（BMSCs）对培养的内皮细胞产生KYN的影响。KYN和色氨酸（TRP）在细胞上清液中的浓度同时测定与高效液相色谱（HPLC）系统配备了荧光检测器（FLD）和紫外检测器（UVD）。我们的研究结果表明，脂多糖刺激的内皮细胞产生更多的KYN，这是伴随着TRP的平行减少。当与BMSC共培养时，与单独脂多糖（LPS）诱导相比，KYN和TRP的产生显着减少。我们的研究结果表明，骨髓基质细胞可以减轻内皮细胞损伤，通过减少KYN检测与HPLC。这种方法是第一种能够捕获细胞中功能变化的方法，简单，快速，适合细胞水平的研究目的。
Endothelial cell dysfunction plays an important role in the occurrence and development of sepsis, which is a consequence of the interaction between coagulation and inflammation. Kynurenine (KYN) is an endothelium-derived relaxing factor that makes a large contribution to sepsis pathophysiology. In this study, we investigated the influence of bone marrow mesenchymal stem cells (BMSCs) on KYN production by cultured endothelial cells. KYN and tryptophan (TRP) concentrations in cell supernatants were simultaneously measured with a high-performance liquid chromatography (HPLC) system equipped with a fluorescence detector (FLD) and an ultraviolet detector (UVD). Our results revealed that lipopolysaccharide-stimulated endothelial cells produced more KYN, which was accompanied by a parallel decrease in TRP. When co-cultured with BMSCs, KYN and TRP production were significantly decreased compared to lipopolysaccharide (LPS)-induction alone. Our results suggest that BMSCs can attenuate endothelial cell damage by decreasing KYN as detected with HPLC. This method is the first to be capable of capturing functional changes in the cells and is simple, fast, and suitable for cellular level research purposes.