HUMAN IG SUPERFAMILY CTLA-4 GENE - CHROMOSOMAL LOCALIZATION AND IDENTITY OF PROTEIN-SEQUENCE BETWEEN MURINE AND HUMAN CTLA-4 CYTOPLASMIC DOMAINS
HUMAN IG SUPERFAMILY CTLA-4 GENE - CHROMOSOMAL LOCALIZATION AND IDENTITY OF PROTEIN-SEQUENCE BETWEEN MURINE AND HUMAN CTLA-4 CYTOPLASMIC DOMAINS
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DOI：
10.1002/eji.1830181206
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发表时间：
1988-12-01
影响因子：
5.4
通讯作者：
LEFRANC, MP
中科院分区：
文献类型：
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作者：
DARIAVACH, P;MATTEI, MG;LEFRANC, MP
小鼠CTLA-4基因已显示编码属于IG基因超家族的活化淋巴细胞相关序列。我们现在报告从基因组文库中分离的人类相应基因的分子克隆和研究，并命名为Hu-CTLA-4。Hu-CTLA-4基因在每个人类单倍体基因组中以单拷贝存在，并定位于2号染色体的q33带。它包含3个外显子，尽管前导序列。第一外显子编码116个氨基酸的V样结构域，第二外显子编码37个氨基酸的疏水性跨膜区，第三外显子编码34个氨基酸的胞质结构域。尽管人和鼠CTLA-4蛋白之间的总体同源性为76%，但值得注意的是，它们的胞质结构域完全相同。这种完整的种间保护支持该结构域在CTLA-4功能中的重要作用。
The mouse CTLA-4 gene has been shown to code for an activated lymphocyte associated sequence belonging to the Ig gene superfamily. We now report on the molecular cloning and study of the human corresponding gene isolated from a genomic library and designated Hu-CTLA-4. The Hu-CTLA-4 gene exists as a single copy per human haploid genome and maps to band q33 of chromosome 2. It comprises 3 exons notwithstanding the leader sequence. The first exon encodes a V-like domain of 116 amino acids, the second one a hydrophobic putative transmembrane region of 37 amino acids and the third one a 34 amino acid putative cytoplasmic domain. Whereas the overall homology between the human and murine CTLA-4 proteins is 76%, there is, remarkably, a complete identity of their cytoplasmic domains. This complete interspecies conservation comes in support of an important role for this domain in CTLA-4 function.