Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH.
Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH.
复制标题
FISH 技术对智利大麦、大麦和普通小麦 3 个材料的个体染色体进行鉴定和比较。
作者：
Rey MD
对3个辣椒大麦材料(H1、H16、H7)、普通大麦和普通小麦的核型进行了物理作图分析。用14个重复序列作为荧光原位杂交(FISH)的探针，目的是鉴定种间和种内的多态性。(AG)12和4P6探针仅在小麦中产生杂交信号，而BAC7探针仅杂交到h着丝粒区域。而pSc119.2探针与小麦和小麦杂交。辣椒，但不是Toh。粗俗。这项研究中使用的其余重复序列在所有基因型中都产生了杂交信号。探针pAs1、PTA-、pTa71、CCS1和CRW非常保守，在所研究的基因型间没有表现出明显的多态性。探针GAA、(AAc)5、(CTA)5、HvT01和pTa794的杂交图谱差异最大。我们在这三个材料中发现了较大的多态性。儿童被研究，支持在物种内部存在不同的类群的建议。辣椒。这项工作中描述的一组探针可以识别所有三个物种的每一条染色体，提供了一个完整的h.Chilense、粗毛小蜂H.Aestivum染色体，这可能在小麦和三叶草育种计划中有用。
Karyotypes of three accessions ofHordeum chilense(H1, H16, and H7),Hordeum vulgare, andTriticum aestivumwere characterized by physical mapping of several repetitive sequences. A total of 14 repetitive sequences were used as probes for fluorescence in situ hybridization (FISH) with the aim of identifying inter- and intraspecies polymorphisms. The (AG)12and 4P6 probes only produced hybridization signals in wheat, the BAC7 probe only hybridized to the centromeric region ofH. vulgare, and the pSc119.2 probe hybridized to both wheat andH. chilense, but not toH. vulgare. The remaining repetitive sequences used in this study produced a hybridization signal in all the genotypes. Probes pAs1, pTa-535, pTa71, CCS1, and CRW were much conserved, showing no significant polymorphism among the genotypes studied. Probes GAA, (AAC)5, (CTA)5, HvT01, and pTa794 produced the most different hybridization pattern. We identified large polymorphisms in the three accessions ofH. chilensestudied, supporting the proposal of the existence of different groups inside species ofH. chilense. The set of probes described in this work allowed the identification of every single chromosome in all three species, providing a complete cytogenetic karyotype ofH. chilense,H. vulgare, andT. aestivumchromosomes, which could be useful in wheat and tritordeum breeding programs.