Safflower Yellow reduce lipid peroxidation, neuropathology, tau phosphorylation and improves amyloid β (1-42)-induced learning and memory impairment in rats
Safflower Yellow reduce lipid peroxidation, neuropathology, tau phosphorylation and improves amyloid β (1-42)-induced learning and memory impairment in rats
复制标题
红花黄减少脂质过氧化、神经病理学、tau 磷酸化并改善淀粉样蛋白 β (1-42) 诱导的大鼠学习和记忆障碍
DOI：
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发表时间：
2015
影响因子：
7.5
通讯作者：
Yan-li Hu
中科院分区：
文献类型：
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作者：
Qin Ma;Ying-ying Ruan;Hui Xu;XuXiao-meng Shi;Yan-li Hu
淀粉样蛋白B蛋白（Ab）的不溶性斑块和过度磷酸化tau的神经元缠结是阿尔茨海默病（AD）的关键标志物。红花黄色素（SY）是从红花中提取的一种中药，被认为具有治疗神经退行性疾病的潜力。然而，SY是否能改善AD模型的学习记忆障碍，其作用机制尚不清楚。在这里，我们给注射淀粉样蛋白B（1-42）的Wistar大鼠灌胃不同剂量的SY 1个月。通过Morris水迷宫实验，我们发现SY治疗显著减轻淀粉样蛋白B（1-42）诱导的大鼠记忆障碍。SY治疗可提高AD大鼠脑组织超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的含量，降低丙二醛（MDA）和乙酰胆碱酯酶（T-CHE）的含量。病理学分析显示SY治疗可抑制注射淀粉样蛋白B（1-42）诱导的皮层和海马神经元形态学改变和tau蛋白过度磷酸化。此外，SY治疗抑制了AD大鼠中上调的CDK-5和GSK-3信号通路。我们的数据表明，红花黄色素可以作为治疗阿尔茨海默病的候选药物。
Insoluble plaques of amyloid b proteins (Ab) and neurofibrillary tangles of hyperphosphorylated tau are key markers for Alzheimer’s disease (AD). Safflower yellow (SY) is one of traditional Chinese medicine extracted from safflower, which is suggested to have therapeutic potential for neurodegenerative.disorders. However, whether SY can ameliorate impairment of learning and memory in AD model, and its causal mechanism are still unclear. Here, we applied different doses of SY intragastrically to Wistar rats injected with amyloid b (1–42) for 1 month. By the Morris water maze test, we found that treatment of SY significantly attenuated amyloid b (1–42)-induced impairment of memory in rats. Mechanistically, SY treatment increased the level of superoxidedismutase (SOD) and Glutathione peroxidase (GSH-Px), and decreased the level of malondialdehyde (MDA) and acetylcholinesterase (T-CHE) in brain tissues of AD rats. Pathological analysis also showed that SY treatment inhibited the morphological alteration of neurons and tau hyperphosphorylation induced by amyloid b (1–42)-injection in the cortex and hippocampus. Moreover, SY treatment inhibited CDK-5 and GSK-3 signaling pathways, which are upregulated in AD rats. Our data indicate that safflower yellow can serve as a therapeutic candidate for Alzheimer’s disease.