Enhanced Ocular Surface and Intraoral Nociception via a Transient Receptor Potential Vanilloid 1 Mechanism in a Rat Model of Obstructive Sleep Apnea
Enhanced Ocular Surface and Intraoral Nociception via a Transient Receptor Potential Vanilloid 1 Mechanism in a Rat Model of Obstructive Sleep Apnea
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DOI：
10.1016/j.neuroscience.2021.12.002
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发表时间：
2022-02-07
期刊：
影响因子：
3.3
通讯作者：
Kato, Takafumi
中科院分区：
文献类型：
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作者：
Kishimoto, Saki;Katagiri, Ayano;Kato, Takafumi
阻塞性睡眠呼吸暂停（OSA）的特征是睡眠期间动脉血氧饱和度低，与口面疼痛的风险增加有关。在这项研究中，我们模拟慢性间歇性缺氧（CIH）在睡眠/休息阶段（光相），以确定瞬时受体电位香草酸1（TRPV 1）介导增强口面伤害性行为和三叉神经脊髓尾侧亚核（Vc）神经元反应辣椒素（TRPV 1激动剂）刺激OSA大鼠模型的作用。大鼠在光照期连续8或16天接受CIH（最低O2，5%）。CIH产生增强的行为反应辣椒素后，应用于眼表面和口腔粘膜，这是逆转在常氧条件下。TRPV 1免疫反应阳性的三叉神经节神经元的百分比在CIH大鼠比在常氧大鼠和CIH后恢复在常氧条件下。CIH大鼠三叉神经节TRPV 1免疫反应阳性神经元的比例增加。CIH大鼠Vc浅层TRPV 1阳性初级传入终末密度增高。磷酸化的细胞外信号调节激酶（pERK）的免疫反应细胞与中央终端的TRPV 1阳性传入的Vc。低剂量辣椒素（0.33 mM）应用于舌后的pERK免疫反应细胞的数量显着大于CIH大鼠的Vc的中间部分比常氧大鼠和CIH后常氧条件下恢复。这些数据表明，CIH在睡眠（轻）阶段是足以通过TRPV 1依赖性机制短暂增强眼表和口腔内粘膜的疼痛。(c)2021作者由Elsevier Ltd代表IBRO出版。
Obstructive sleep apnea (OSA), characterized by low arterial oxygen saturation during sleep, is associated with an increased risk of orofacial pain. In this study, we simulated chronic intermittent hypoxia (CIH) during the sleep/rest phase (light phase) to determine the role of transient receptor potential vanilloid 1 (TRPV1) in mediating enhanced orofacial nocifensive behavior and trigeminal spinal subnucleus caudalis (Vc) neuronal responses to capsaicin (a TRPV1 agonist) stimulation in a rat model of OSA. Rats were subjected to CIH (nadir O2, 5%) during the light phase for 8 or 16 consecutive days. CIH yielded enhanced behavioral responses to capsaicin after application to the ocular surface and intraoral mucosa, which was reversed under normoxic conditions. The percentage of TRPV1-immunoreactive trigeminal ganglion neurons was greater in CIH rats than in normoxic rats and recovered under normoxic conditions after CIH. The ratio of large-sized TRPV1immunoreactive trigeminal ganglion neurons increased in CIH rats. The density of TRPV1 positive primary afferent terminals in the superficial laminae of Vc was higher in CIH rats. Phosphorylated extracellular signal-regulated kinase (pERK)-immunoreactive cells intermingled with the central terminal of TRPV1-positive afferents in the Vc. The number of pERK-immunoreactive cells following low-dose capsaicin (0.33 mM) application to the tongue was significantly greater in the middle portion of the Vc of CIH rats than of normoxic rats and recovered under normoxic conditions after CIH. These data suggest that CIH during the sleep (light) phase is sufficient to transiently enhance pain on the ocular surface and intraoral mucosa via TRPV1-dependent mechanisms.(c) 2021 The Authors. Published by Elsevier Ltd on behalf of IBRO.