RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development.
RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development.
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RNA结合蛋白MAC5A是赤霉素调节雄蕊发育所必需的
DOI：
10.3390/ijms23042009
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发表时间：
2022-02-11
影响因子：
5.6
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Liu H;Shang H;Yang H;Liu W;Tsugama D;Nonomura KI;Zhou A;Wu W;Takano T;Liu S
花器官的发育由一个复杂的同源异源基因网络来协调，赤霉素(GA)信号参与花器官的发育；然而，潜在的分子机制仍然不清楚。在本研究中，我们发现MOS4相关复合体5A(MAC5A)是一种含有RNA结合基序的蛋白质，参与了萼片、花瓣和雄蕊的发育；MAC5A功能的丧失或获得都会导致雄蕊畸形和结实率降低。外源GA的应用大大加剧了mac5a缺失突变体的缺陷，包括雄蕊减少和雄性不育。MAC5A主要在花粉粒和雄蕊中表达，MAC5A的过度表达影响APETALA1(AP1)、AP2和AGAMOUS(AG)等同源基因的表达。MAC5A可能与兔耳(RBE)相互作用，RBE是拟南芥花中AG表达的抑制因子。Rbe缺失突变体的花瓣缺陷至少在mac5a rbe双突变体中得到了部分挽救。这些发现表明，MAC5A是一个新的因子，它是雄蕊正常发育所必需的，并依赖于GA信号通路。
The development of floral organs is coordinated by an elaborate network of homeotic genes, and gibberellin (GA) signaling is involved in floral organ development; however, the underlying molecular mechanisms remain elusive. In the present study, we found that MOS4-ASSOCIATED COMPLEX 5A (MAC5A), which is a protein containing an RNA-binding motif, was involved in the development of sepals, petals, and stamens; either the loss or gain of MAC5A function resulted in stamen malformation and a reduced seed set. The exogenous application of GA considerably exacerbated the defects in mac5a null mutants, including fewer stamens and male sterility. MAC5A was predominantly expressed in pollen grains and stamens, and overexpression of MAC5A affected the expression of homeotic genes such as APETALA1 (AP1), AP2, and AGAMOUS (AG). MAC5A may interact with RABBIT EARS (RBE), a repressor of AG expression in Arabidopsis flowers. The petal defect in rbe null mutants was at least partly rescued in mac5a rbe double mutants. These findings suggest that MAC5A is a novel factor that is required for the normal development of stamens and depends on the GA signaling pathway.
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