Involvement of non-coding RNAs during infection of rice by Acidovorax oryzae
Involvement of non-coding RNAs during infection of rice by Acidovorax oryzae
复制标题
非编码 RNA 在水稻食酸菌感染过程中的参与
DOI：
10.1111/1758-2229.12982
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发表时间：
2021-06-13
影响因子：
3.3
通讯作者：
Li，Bin
中科院分区：
文献类型：
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作者：
Cui，Zhouqi;Zhang，Yang;Li，Bin
已在多种细菌中观察到非编码RNA（ncRNA）的表达。然而，ncRNA及其调控靶点的功能在很大程度上是未知的，并且很少发现ncRNA与细菌毒力相关。细菌褐色条纹病原体Acidovorax peptide（Ao）RS-1显示出高水平的ncRNA的条件依赖性差异表达，这是我们在全基因组筛选中鉴定的。我们通过整合分析稻瘟病菌与水稻的体内相互作用过程中的保守基因组序列和转录组数据，实验验证了66个差异表达的ncRNA。为了测试差异表达的ncRNA的相关性，我们选择了四个在基因组内具有不同位置，并且具有不同二级结构和启动子活性的ncRNA。结果表明，4种ncRNA的过表达引起了毒力相关表型、对各种环境胁迫的抗性、分泌系统和效应蛋白的表达的显著变化，同时改变了ncRNA推定靶基因的表达。我们的结论是，这些ncRNA是许多观察到的ncRNA响应于不断变化的条件，如主机相互作用或环境适应的固有调节作用的例子。
The expression of non‐coding RNAs (ncRNAs) has been observed in a variety of bacteria. However, the function of ncRNAs and their regulatory targets are largely unknown, and few ncRNAs are found to be associated with bacterial virulence. The bacterial brown stripe pathogenAcidovorax oryzae(Ao) RS‐1 shows a high level of condition‐dependent differential expression of ncRNA, which we identified in a genome wide screen. We experimentally validated 66 differentially expressed ncRNAs using an integrative analysis of conservative genome sequences and transcriptomic data duringin vivointeraction of the bacterial pathogen with the rice plant. To test the relevance of the differentially expressed ncRNAs, we chose four with different positions within the genome, and with different secondary structures and promoter activities. The results show that the overexpression of the four ncRNAs caused a significant change in virulence‐related phenotypes, resistance to various environmental stresses, expression of secretion systems and effector proteins, while changing the expression of ncRNA putative target genes. We conclude that these ncRNAs are examples for the inherent regulatory roles for many of the observed ncRNAs in response to changing conditions such as host interaction or environmental adaption.