A NEW GENUS OF THE ORDER ACTINOMYCETALES, COUCHIOPLANES GEN-NOV, WITH DESCRIPTION OF COUCHIOPLANES CAERULEUS (HORAN AND BRODSKY 1986) COMB-NOV AND COUCHIOPLANES CAERULEUS SUBSP AZUREUS SUBSP NOV
A NEW GENUS OF THE ORDER ACTINOMYCETALES, COUCHIOPLANES GEN-NOV, WITH DESCRIPTION OF COUCHIOPLANES CAERULEUS (HORAN AND BRODSKY 1986) COMB-NOV AND COUCHIOPLANES CAERULEUS SUBSP AZUREUS SUBSP NOV
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DOI：
10.1099/00207713-44-2-193
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发表时间：
1994-04-01
期刊：
影响因子：
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通讯作者：
YOKOTA, A
中科院分区：
文献类型：
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作者：
TAMURA, T;NAKAGAITO, Y;YOKOTA, A
在我们对运动放线菌的分类学研究中，发现土壤分离株 RA 335 形成蓝色底物菌丝体和带有运动节孢子的气生菌丝体，并含有赖氨酸作为细胞壁二氨基酸。 Actinoplanes caeruleus IFO 13939(T)（T = 型菌株）和“Actinoplanes azureus”IFO 13993(T) 已知具有相同的特征。因此，对这三个菌株的分类地位进行了研究。这些菌株的气生菌丝体在生长周期中破碎，并产生在菌丝体内呈链状排列的活动孢子。未观察到孢子囊。该菌株含有甲基萘醌9(H-4)，鸟嘌呤加胞嘧啶含量为69.9至72.1mol%，细胞壁氨基酸为D-谷氨酸、D-和L-丝氨酸、甘氨酸、L-丙氨酸和L-赖氨酸(A3α型)。这些菌株的分类学特征与先前描述的运动放线菌的分类学特征不同。根据形态学、生理学和化学分类学数据以及 DNA-DNA 杂交和 16S rRNA 比较研究的结果，我们为这些生物提出了一个新属：Couchioplanes。模式种是Couchioplanes caeruleus come。十一月（模式菌株，IFO 13939），分为两个亚种，Couchioplanes caeruleus subsp。蓝藻亚种十一月（模式菌株，IFO 13939）A. caeruleus IFO 13939(T) 和菌株 RA 335 以及 Couchioplanes caeruleus subsp.（模式菌株，IFO 13939）。 azureus（典型菌株，IFO 13993）为“A”。 azureus”IFO 13993(T)。
During our taxonomic study of motile actinomycetes, soil isolate RA 335 was found to form a blue substrate mycelium and aerial mycelia with motile arthrospores and to have lysine as the cell wall diamino acid. Actinoplanes caeruleus IFO 13939(T) (T = type strain) and ''Actinoplanes azureus'' IFO 13993(T) are known to have the same characteristics. Therefore, the taxonomic position of these three strains was studied. Aerial mycelia of these strains fragmented during the growth cycle and produced motile spores arranged in chains within the mycelia. Sporangia were not observed. The strains contained menaquinone 9(H-4), had guanine-plus-cytosine contents of 69.9 to 72.1 mol%, and had D-glutamic acid, D- and L-serine, glycine, L-alanine, and L-lysine as cell wall amino acids (type A3 alpha). The taxonomic characteristics of these strains differ from those of the previously described motile actinomycetes. On the basis of morphological, physiological, and chemotaxonomic data and the results of DNA-DNA hybridization and comparative 16S rRNA studies, we propose a new genus, Couchioplanes, for these organisms. The type species is Couchioplanes caeruleus comb. nov. (type strain, IFO 13939), which is divided into two subspecies, Couchioplanes caeruleus subsp. caeruleus subsp. nov. (type strain, IFO 13939) for A. caeruleus IFO 13939(T) and strain RA 335 and Couchioplanes caeruleus subsp. azureus (type strain, IFO 13993) for ''A. azureus'' IFO 13993(T).