DISTINCT ROLES FOR CYCLIN-DEPENDENT KINASES IN CELL-CYCLE CONTROL
DISTINCT ROLES FOR CYCLIN-DEPENDENT KINASES IN CELL-CYCLE CONTROL
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DOI：
10.1126/science.8266103
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发表时间：
1993-12-24
期刊：
影响因子：
56.9
通讯作者：
HARLOW, E
中科院分区：
文献类型：
--
作者：
VANDENHEUVEL, S;HARLOW, E
关键的细胞周期调节因子 Cdc2 属于高等真核生物中的细胞周期蛋白依赖性激酶家族。显性失活突变用于解决人类细胞周期进展中对该家族激酶的需求。显性失活的 Cdc2 突变体使细胞停滞在 G2 到 M 期的转变，而细胞周期蛋白依赖性激酶 Cdk2 和 Cdk3 的突变体则引起 G1 阻滞。突变表型被相应的野生型激酶特异性拯救。这些数据表明，除了 Cdc2 和 Cdk2 之外，Cdk3 在哺乳动物细胞周期中执行独特且重要的功能。
The key cell-cycle regulator Cdc2 belongs to a family of cyclin-dependent kinases in higher eukaryotes. Dominant-negative mutations were used to address the requirement for kinases of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant arrested cells at the G2 to M phase transition, whereas mutants of the cyclin-dependent kinases Cdk2 and Cdk3 caused a G1 block. The mutant phenotypes were specifically rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to Cdc2 and Cdk2, executes a distinct and essential function in the mammalian cell cycle.