INCREASED ACTIVITY OF THE EEF-2 SPECIFIC, CA2+ AND CALMODULIN DEPENDENT PROTEIN-KINASE III DURING THE S-PHASE IN EHRLICH ASCITES-CELLS
INCREASED ACTIVITY OF THE EEF-2 SPECIFIC, CA2+ AND CALMODULIN DEPENDENT PROTEIN-KINASE III DURING THE S-PHASE IN EHRLICH ASCITES-CELLS
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DOI：
10.1016/s0006-291x(05)81347-6
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发表时间：
1991-11-14
影响因子：
3.1
通讯作者：
NYGARD, O
中科院分区：
文献类型：
--
作者：
CARLBERG, U;NILSSON, A;NYGARD, O
研究了匀浆埃利希腹水肿瘤细胞中真核延伸因子（eEF-2）特异性、Ca2+和钙调蛋白依赖性蛋白激酶III （CaM PK III）的活性。eEF-2激酶活性是在底物过量的情况下测定的，即eEF-2的非极限浓度。匀浆显示激酶和磷酸酶活性。后者的活性受到蛋白磷酸酶2A抑制剂冈田酸的抑制。对细胞周期特定阶段的细胞进行的激酶分析表明，s期早期的细胞活性最高，而G2+M期的磷酸酶活性最明显。
The activity of the eukaryotic elongation factor (eEF-2) specific, Ca2+and calmodulin dependent protein kinase III (CaM PK III) was studied in homogenated Ehrlich ascites tumour cells. The eEF-2 kinase activity was determined in the presence of an excess of the substrate, i.e., non-limiting concentrations of eEF-2. The homogenates showed both kinase and phosphatase activity. The latter activity was inhibited by the protein phosphatase 2A inhibitor okadaic acid. Analysis of the kinase using cells from defined stages of the cell cycle showed that the highest activity was found in cells from the early S-phase, whereas the phosphatase activity was most pronounced during the G2+M phase.