New dot-blot method for evaluating the effect of inactivators on mite and Japanese cedar pollen allergens
New dot-blot method for evaluating the effect of inactivators on mite and Japanese cedar pollen allergens
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用于评估灭活剂对螨虫和日本柳杉花粉过敏原影响的新斑点印迹方法
DOI：
10.1093/bbb/zbab146
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sakaguchi Masahiro
中科院分区：
文献类型：
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作者：
Yoshida Megumi;Mizukami Keijiro;Kurata Keigo;Nasukawa Tadahiro;Uchiyama Jumpei;Sakaguchi Masahiro
作为一种评价灭活剂对过敏原的影响，同时抑制灭活剂对测定的影响的方法，我们开发了新的斑点印迹法，结合免疫染色和蛋白质检测方法。该方法可用于评估灭活剂是否能杀灭过敏原，而不是将其从测定中去除。
As a method of evaluating the effect of inactivators on allergens while suppressing the effect of inactivator on the assay, we developed new dot-blot method that combines immunostaining and protein detection methods. This method is useful for evaluating whether the inactivator can inactivate allergens rather than removing them from the assay.