A web-based tool for rational screening of mutants libraries using ProSAR
A web-based tool for rational screening of mutants libraries using ProSAR
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DOI：
10.1093/protein/gzu035
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发表时间：
2014-10-01
影响因子：
2.4
通讯作者：
Charton, Philippe
中科院分区：
文献类型：
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作者：
Berland, Magali;Offmann, Bernard;Charton, Philippe
在定向进化实验中，有效筛选突变体文库的方法至关重要。我们提出了一种基于网络的工具，该工具实施已建立的计算机方法来合理筛选突变体文库。该方法称为 ProSAR，试图将序列数据与活动联系起来。该方法使用在用户提供的小型实验数据集上训练的统计模型。这些可以整合突变之间潜在的上位相互作用，并可用于许多不同的生物环境。它极大地改善了对主要突变体的搜索。该工具可免费提供给非商业用户，网址为 。
In directed evolution experiments, it is at stake to have methods to screen efficiently the mutant libraries. We propose a web-based tool that implements an established in silico method for the rational screening of mutant libraries. The method, known as ProSAR, attempts to link sequence data to activity. The method uses statistical models trained on small experimental datasets provided by the user. These can integrate potential epistatic interactions between mutations and be used in many diverse biological contexts. It drastically improves the search for leading mutants. The tool is freely available to non-commercial users at .