Development of taxon-specific markers for high-throughput screening of microbial-feeding nematodes
Development of taxon-specific markers for high-throughput screening of microbial-feeding nematodes
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DOI：
10.1111/j.1471-8286.2006.01319.x
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发表时间：
2006-09-01
期刊：
影响因子：
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通讯作者：
Herman, Michael A.
中科院分区：
文献类型：
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作者：
Jones, Kenneth L.;Todd, Timothy C.;Herman, Michael A.
在努力评估的分类身份的大规模采样的线虫从Konza高草草原，我们测序的18S rRNA基因的一部分，其相关的内部转录间隔区（ITS1）从74线虫包括4个分类家庭。从这些序列中，我们已经开发了一系列的分子探针来区分16个不同的食微生物线虫类群。使用低倍显微镜和分类特异性实时探针的组合，74线虫被正确地分配到各自的分类组。这种优化的方法提供了一种高通量测定，以确定线虫的身份在更大的数据集。
In an effort to assess the taxonomic identity of large-scale samplings of nematodes from the Konza Tallgrass Prairie, we sequenced a portion of the 18S rRNA gene and its associated internally transcribed spacer (ITS1) from 74 nematodes encompassing four taxonomic families. From these sequences, we have developed a series of molecular probes to distinguish 16 distinct microbivorous nematode taxa. Using a combination of low power microscopy and taxon-specific real-time probes, the 74 nematodes were correctly assigned to their respective taxonomic groups. This optimized method provides a high-throughput assay to determine nematode identities across larger data sets.