Micro-CT observation of angiogenesis in bone regeneration
Micro-CT observation of angiogenesis in bone regeneration
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DOI：
10.1111/j.1600-0501.2012.02458.x
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发表时间：
2013-07-01
影响因子：
4.3
通讯作者：
Ito, Koichi
中科院分区：
文献类型：
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作者：
Udagawa, Asami;Sato, Shuichi;Ito, Koichi
目的恢复足够的血供是骨愈合过程中必不可少的，也是骨增量手术成功的关键。在这项研究中，我们评估了在大鼠颅骨扁平骨缺损，在体内微焦点计算机断层扫描（micro-CT）的血管生成。材料与方法20只大鼠。暴露颅骨，并制备临界（直径5 mm）和非临界（直径2.7 mm）尺寸的颅骨骨缺损。通过显微CT图像分析和组织学检查评价骨再生和血管生成。结果颅骨临界和非临界骨缺损均表现为矢状缝周围骨再生和新生血管形成。在第28天，临界尺寸的颅骨缺损显示原始手术缺损的约1.2%的再骨化，而非临界尺寸的缺损显示约43.3%的再骨化。此外，在临界尺寸的颅骨骨缺损中观察到血管生成较晚（约38.2%），而在第28天的非临界尺寸的颅骨骨缺损中观察到血管生成较早（约75.5%）。在两种尺寸的缺损周围观察到新的血管网络。结论在颅骨缺损的临界和非临界范围内，血管生成先于骨再生。血管生成导致非临界尺寸缺损的完全骨形成。
Objectives Restoration of an adequate blood supply is essential for the bone healing process and is key to the success of bone augmentation procedures. In this study, we evaluated angiogenesis in rat calvarial flat bone defects using in vivo microfocus computed tomography (micro-CT). Materials and methods Twenty rats were used. The calvarium was exposed and calvarial bone defects of critical (5-mm diameter) and non-critical (2.7-mm diameter) sizes were prepared. Bone regeneration and angiogenesis were evaluated by image analysis using micro-CT and histological examination. Results Critical- and non-critical-sized calvarial bone defects showed bone regeneration and angiogenesis around the midsagittal suture. Critical-sized calvarial bone defects showed approximately 1.2% reossification of the original surgical defect, whereas the non-critical-sized defects showed approximately 43.3% reossification at day 28. Furthermore, angiogenesis was observed later in the critical-sized calvarial bone defects (about 38.2%), whereas angiogenesis was observed early in the non-critical-sized calvarial bone defects (about 75.5%) at day 28. New blood vessel networks were observed around defects of both sizes. Conclusions Angiogenesis preceded bone regeneration around critical- and non-critical-sized calvarial bone defects. Angiogenesis led to full bone formation in non-critical-sized defects.