PURIFICATION AND CHARACTERIZATION OF A HOST-SELECTIVE NECROSIS TOXIN FROM PYRENOPHORA-TRITICI-REPENTIS
PURIFICATION AND CHARACTERIZATION OF A HOST-SELECTIVE NECROSIS TOXIN FROM PYRENOPHORA-TRITICI-REPENTIS
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DOI：
10.1016/0885-5765(89)90051-9
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发表时间：
1989-09-01
影响因子：
2.7
通讯作者：
BERNIER, CC
中科院分区：
文献类型：
--
作者：
BALLANCE, GM;LAMARI, L;BERNIER, CC
小麦核腔菌（Pyrenophora tritici-repentis）的分离株是小麦褐斑病的病原菌，在适当的小麦分化上仅引起坏死、仅引起失绿或坏死和失绿作为感染症状。毒素已被纯化，从培养滤液的坏死，只有隔离。该毒素渗入感病品种的叶片可引起叶片坏死，但对抗病品种无影响。通过凝胶过滤和离子交换层析纯化毒素，并显示为蛋白质。使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示毒素纯度，并估计蛋白质分子量为13，900。测定了氨基酸组成和摩尔消光。蛋白水解消化或在变性条件下处理纯化的毒素破坏毒素活性。将10-13-10-14摩尔纯化毒素渗入感病品种中会产生坏死。
Isolates of Pyrenophora tritici-repentis, the causal agent of tan spot, cause necrosis only, chlorosis only or necrosis and chlorosis as symptoms of infection on appropriate wheat differentials. A toxin has been purified from a culture filtrate of a necrosis-only isolate. The toxin produces necrosis if it is infiltrated into the leaves of cultivars susceptible to the isolate but is without effect on cultivars resistant to the isolate. The toxin was purified by gel filtration and ion exchange chromatography and shown to be a protein. Sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to show the toxin purity as well as estimate a protein molecular weight of 13,900. The amino acid composition and molar extinction were determined. Proteolytic digestion or treatment of the purified toxin under denaturing conditions destroyed toxin activity. Infiltration of 10-13-10-14 moles of purified toxin into susceptible cultivars will produce necrosis.