Dynamic Characteristics of Sequential Acute Exacerbations and Risk Windows in AECOPD Rats Induced by Cigarette-Smoke and Exposure to Klebsiella pneumoniae.
Dynamic Characteristics of Sequential Acute Exacerbations and Risk Windows in AECOPD Rats Induced by Cigarette-Smoke and Exposure to Klebsiella pneumoniae.
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DOI：
10.1248/bpb.b18-00148
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发表时间：
2018-10
影响因子：
2
通讯作者：
Jiansheng Li;Ya Li;Xiao-fan Lu;Haifeng Wang;Yang Wang;Hangjie Li;Zhaohuan Wu
中科院分区：
文献类型：
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作者：
Jiansheng Li;Ya Li;Xiao-fan Lu;Haifeng Wang;Yang Wang;Hangjie Li;Zhaohuan Wu
慢性阻塞性肺疾病（COPD）的风险窗（RW）是指急性加重（AE）后至稳定期前的一段时间，在此期间急性加重易复发。我们建立了一个连续的COPD-AE-RW大鼠模型，前8周采用香烟烟雾和细菌暴露，并在第9周第1天用肺炎克雷伯菌攻击以模拟AE，发现AECOPD大鼠在攻击后24 h体温、白色血细胞、中性粒细胞、血清淀粉样蛋白A（SAA）和C反应蛋白（CRP）升高，3-6 d下降，肺功能48 h下降，7-16 d恢复。处死时，肺用力呼气量（FEV）100和FEV 300下降，同时观察到支气管肺泡灌洗液（BALF）中性粒细胞升高和明显的气道炎症、重塑和肺气肿。成功建立了COPD-AE-RW大鼠序贯模型，AE期持续约5-7 d，随后为10 d左右的风险窗。
The risk-window (RW) of chronic obstructive pulmonary disease (COPD) is a period after an acute exacerbation (AE) but before the following stable phase, in which exacerbations are easy to relapse. We established a sequential COPD-AE-RW rat model by cigarette-smoke and bacterial exposures in the first 8 weeks, and was challenged with Klebsiella pneumonia to mimic an AE on Day 1 of week 9, and found that body temperature, white blood cell, neutrophils, serum amyloid A (SAA) and C-reactive protein (CRP) increased in AECOPD rats 24 h after challenge, and declined in 3-6 d, while lung function declined in 48 h, and recovered in 7-16 d. When sacrificed, pulmonary forced expiratory volume (FEV)100 and FEV300 decreased, while elevated bronchoalveolar lavage fluid (BALF) neutrophils and marked airway inflammation, remodeling and emphysema were observed. Sequential COPD-AE-RW rat model was established successfully and AE phase lasts for approximately 5-7 d, followed by a 10-d around risk-window.