Chryseomicrobium imtechense gen. nov., sp nov., a new member of the family Planococcaceae
Chryseomicrobium imtechense gen. nov., sp nov., a new member of the family Planococcaceae
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DOI：
10.1099/ijs.0.023184-0
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发表时间：
2011-08-01
影响因子：
2.8
通讯作者：
Jain, Rakesh Kumar
中科院分区：
文献类型：
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作者：
Arora, Pankaj Kumar;Chauhan, Archana;Jain, Rakesh Kumar
从印度孟加拉湾海水中分离到一株革兰氏染色阳性、棒状、黄色、不运动、不产芽孢的严格需氧菌株，命名为mw10 (T)，并进行了多相分类研究。16S rRNA基因序列分析显示，菌株mw10t与嗜冷冷杆菌（Psychrobacillus psychrodurans）和耐冷冷杆菌（Psychrobacillus psych耐受性）型菌株的相似性最高（96.15%），与Paenisporosarcina、Planococcus、Sporosarcina和Planomicrobium属成员的相似性小于96%。基于16S rRNA基因序列的系统发育分析表明，菌株mw10 (T)与近缘属成员形成一个分离的分支。菌株mw10 (T)的形态、生理和化学分类特征与近缘属成员不同。菌株mw10 (T)的主要脂肪酸为iso-C-15:0，甲基萘醌类为MK-7（48.4%）、MK-8（32.3%）、MK-7(H-2)（13.7%）和MK-6（5.6%）。极性脂质为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、一种未知磷脂、一种未知脂质和一种未知糖脂。细胞壁肽聚糖类型为L-Lys-D-Asp。菌株mw10 (T)基因组DNA G+C含量（53.4 mol%）与近缘属成员差异显著。根据菌株mw10 (T)的形态、生理和化学分类特征以及系统发育分析，我们认为菌株mw10 (T)是一个新的属和种，并建议将其命名为Chryseomicrobium imtechense gen. nov., sp. nov.。菌株类型为mw10 (T) (=MTCC 10098（T) =JCM 16573(T)）。
A Gram-stain-positive, rod-shaped, yellow, non-motile, non-spore-forming, strictly aerobic bacterial strain, designated MW 10(T), was isolated from seawater of the Bay of Bengal, India, and was subjected to a polyphasic taxonomic study. Analysis of the 16S rRNA gene sequence revealed that strain MW 10T showed highest similarity to the type strains of Psychrobacillus psychrodurans (96.15%) and Psychrobacillus psychrotolerans (96.01%) and showed less than 96% similarity to members of the genera Paenisporosarcina, Planococcus, Sporosarcina and Planomicrobium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain MW 10(T) formed a clade separate from members of closely related genera. The morphological, physiological and chemotaxonomic characteristics of strain MW 10(T) differed from those of members of closely related genera. The major fatty acid in strain MW 10(T) was iso-C-15:0 and the menaquinones were MK-7 (48.4%), MK-8 (32.3%), MK-7(H-2) (13.7%) and MK-6 (5.6%). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown phospholipid, an unknown lipid and an unknown glycolipid. The cell-wall peptidoglycan type was L-Lys-D-Asp. The genomic DNA G+C content (53.4 mol%) of strain MW 10(T) was significantly different from those of members of closely related genera. On the basis of its morphological, physiological and chemotaxonomic characteristics as well as our phylogenetic analysis, we conclude that strain MW 10(T) is a member of a novel genus and species, for which the name Chryseomicrobium imtechense gen. nov., sp. nov., is proposed. The type strain of Chryseomicrobium imtechense is MW 10(T) (=MTCC 10098(T) =JCM 16573(T)).