3D printed tissue engineered model for bone invasion of oral cancer
3D printed tissue engineered model for bone invasion of oral cancer
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DOI：
10.1016/j.tice.2018.03.009
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发表时间：
2018-06-01
期刊：
影响因子：
2.6
通讯作者：
Moharamzadeh, Keyvan
中科院分区：
文献类型：
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作者：
Almela, Thafar;Al-Sahaf, Sarmad;Moharamzadeh, Keyvan
三维打印技术的最新进展使其在组织工程中的应用迅速扩大。本研究旨在以3D打印支架为基础，结合组织工程口腔黏膜模型，建立并表征体外多层人牙槽骨。其目的是在不同的解剖水平将口腔鳞状细胞癌(OSCC)细胞系球体合并到3D模型中，以表示口腔癌的不同阶段。3D组织模型的组织学评估显示了一个由不同的上皮、结缔组织和骨层组成的三层结构；复制了正常的口腔组织结构。细胞角蛋白13和14的免疫组织化学显示，黏膜部分显示出与自然组织相似的良好分化的复层口腔鳞状上皮。组织学评估显示，口腔鳞癌模型在上皮上皮上、上皮下水平和结缔组织-骨界面上三个深度处呈球形。3D组织工程化复合模型模拟了自然口腔硬组织和软组织，有望成为研究口腔癌骨侵袭的体外模型，并为未来口腔鳞癌治疗的新的诊断或治疗方法的评估提供参考。
Recent advances in three-dimensional printing technology have led to a rapid expansion of its applications in tissue engineering. The present study was designed to develop and characterize an in vitro multi-layered human alveolar bone, based on a 3D printed scaffold, combined with tissue engineered oral mucosal model. The objective was to incorporate oral squamous cell carcinoma (OSCC) cell line spheroids to the 3D model at different anatomical levels to represent different stages of oral cancer. Histological evaluation of the 3D tissue model revealed a tri-layered structure consisting of distinct epithelial, connective tissue, and bone layers; replicating normal oral tissue architecture. The mucosal part showed a well-differentiated stratified oral squamous epithelium similar to that of the native tissue counterpart, as demonstrated by immunohistochemistry for cytokeratin 13 and 14. Histological assessment of the cancerous models demonstrated OSCC spheroids at three depths including supra-epithelial level, sub-epithelial level, and deep in the connective tissue-bone interface.The 3D tissue engineered composite model closely simulated the native oral hard and soft tissues and has the potential to be used as a valuable in vitro model for the investigation of bone invasion of oral cancer and for the evaluation of novel diagnostic or therapeutic approaches to manage OSCC in the future.