Reconstitution of Sec‐dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG‐dependent manner
Reconstitution of Sec‐dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG‐dependent manner
复制标题
Sec 依赖性膜蛋白插入的重建：新生的 FtsQ 以 SecYEG 依赖性方式与 YidC 相互作用
作者：
Martin van der Laan;E. Houben;N. Nouwen;J. Luirink;A. Driessen
内膜蛋白 YidC 与大肠杆菌的前蛋白转位酶相关，并在膜插入过程中接触新生内膜蛋白的跨膜片段。 YidC 被纯化至同质并与 SecYEG 复合物共重构。 YidC 对 SecA/SecYEG 介导的分泌蛋白 proOmpA 的易位没有影响；然而，使用交联方法，发现新生 FtsQ 的跨膜片段可以通过 SecY 访问 YidC。这些数据表明 YidC 依赖性膜蛋白通过 SecYEG 复合物插入的初始阶段的功能重建。
The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion. YidC was purified to homogeneity and co‐reconstituted with the SecYEG complex. YidC had no effect on the SecA/SecYEG‐mediated translocation of the secretory protein proOmpA; however, using a crosslinking approach, the transmembrane segment of nascent FtsQ was found to gain access to YidC via SecY. These data indicate the functional reconstitution of the initial stages of YidC‐dependent membrane protein insertion via the SecYEG complex.