Antitumor activity with the HSV-tk-gene-modified cell line PA-1-STK in malignant mesothelioma.
Antitumor activity with the HSV-tk-gene-modified cell line PA-1-STK in malignant mesothelioma.
复制标题
HSV-tk 基因修饰细胞系 PA-1-STK 在恶性间皮瘤中的抗肿瘤活性。
DOI：
10.1165/ajrcmb.19.2.3123
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Kolls,JK
中科院分区：
文献类型：
--
作者：
Schwarzenberger,P;Lei,D;Freeman,SM;Ye,P;Weinacker,A;Theodossiou,C;Summer,W;Kolls,JK
Malignant mesothelioma (MM) is a thoracic malignancy that is increasing in incidence. Since it is uniformly fatal and kills by local spread, investigators have proposed that MM is a good target for novel treatment approaches, such as gene therapy. We hypothesized that delivery of the HSV-tk gene, using gene-modified tumor cells (PA-1-STK cells), would result in an antitumor effect after treatment with ganciclovir. Inin vitromixing experiments, we found that PA-1-STK cells killed both mouse and human mesothelioma cells in a dose-dependent manner. Moreover, we found that PA-1-STK cells also prolonged survival of mice with MM when the percentage of total tumor cells was high (70%), but observed no survival benefit when the percentage of PA-1-STK cells was low (30%). These data support the rationale for a cell-based gene therapy approach to MM.
