Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate
Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate
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DOI：
10.1016/j.bbrc.2009.05.117
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发表时间：
2009-08-14
影响因子：
3.1
通讯作者：
Kawai, Toshihisa
中科院分区：
文献类型：
--
作者：
Kajiya, Mikihito;Silva, Marcelo J. B.;Kawai, Toshihisa
据报道，分子氢（H-2）通过其抗氧化作用对缺血再灌注引起的组织损伤具有保护作用。为了评价其抗炎作用，我们通过向小鼠提供含有（1）葡聚糖硫酸钠（DSS）（5%）、（2）DSS（5%）和H-2或（3）仅H-2的水自由饮用长达7天来建立人炎症性肠病（IBD）小鼠模型。在第7天，DSS诱导的致病性结果包括体重减轻、结肠炎评分增加、结肠长度致病性缩短、IL-12水平升高。在DSS溶液中加入H-2可显著抑制结肠病变中的TNF-α和IL-1 β。组织学分析还显示，DSS介导的结肠组织破坏伴随着巨噬细胞浸润显着抑制H-2。因此，本研究表明，H-2可以防止DSS诱导的小鼠结肠炎的发展。(C)2009 Elsevier Inc. All rights reserved.
By its antioxidant effect, molecular hydrogen gas (H-2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H-2, or (3) H-2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12. TNF-alpha and IL-1 beta in colon lesion, were significantly suppressed by the addition of H-2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H-2. Therefore, the present study indicated that H-2 can prevent the development of DSS-induced colitis in mice. (C) 2009 Elsevier Inc. All rights reserved.