Interferon alpha induces protein kinase C-epsilon (PKC-epsilon) gene expression and a 4.7-kb PKC-epsilon-related transcript.
Interferon alpha induces protein kinase C-epsilon (PKC-epsilon) gene expression and a 4.7-kb PKC-epsilon-related transcript.
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干扰素 α 诱导蛋白激酶 C-ε (PKC-ε) 基因表达和 4.7 kb PKC-ε 相关转录物。
DOI：
10.1073/pnas.90.15.6944
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发表时间：
1993
影响因子：
11.1
通讯作者：
Pfeffer,LM
中科院分区：
文献类型：
--
作者：
Wang,C;Constantinescu,SN;MacEwan,DJ;Strulovici,B;Dekker,LV;Parker,PJ;Pfeffer,LM
蛋白激酶在人干扰素α（IFN-α）诱导各种人细胞系中的特异性基因表达和生物活性中起关键作用。我们现在报告，IFN-α增加了7-kb的转录蛋白激酶C（PKC-β）和PKC-β的细胞内容物24和48小时后，IFN-α添加（2倍和6倍的增加，分别）。此外，IFN-α显着诱导的4.7 kb的转录本，杂交的PKC-β-特异性，但不是PKC-β-特异性，cDNA探针。IFN-α诱导4.7-kb PKC-β相关mRNA具有经典IFN-α刺激基因的以下特性：诱导动力学快速，IFN-α敏感但不耐IFN-α的细胞系中维持高水平，蛋白合成独立诱导，对蛋白酪氨酸激酶活性抑制剂的高敏感性。这些结果表明，IFN-α对基因表达的调控不仅包括经典的IFN-α刺激基因，而且还包括与IFN-α的生物学作用高度相关的两个PKC-β相关转录物的协调调控。
Protein kinases play key roles in the induction by human interferon alpha (IFN-alpha) of specific gene expression and biological activity in various human cell lines. We now report that IFN-alpha increased the 7-kb transcript for the epsilon isotype of protein kinase C (PKC-epsilon) and the cellular content of PKC-epsilon 24 and 48 hr after IFN-alpha addition (a 2-fold and 6-fold increase, respectively). Furthermore, IFN-alpha markedly induced a 4.7-kb transcript that hybridized to a PKC-epsilon-specific, but not to a PKC-eta-specific, cDNA probe. The induction of the 4.7-kb PKC-epsilon-related mRNA by IFN-alpha had the following properties reported for the classical IFN-alpha-stimulated genes: rapid kinetics of induction, high maintained levels in IFN-alpha-sensitive but not in IFN-alpha-resistant cell lines, protein synthesis-independent induction, and high sensitivity to inhibitors of protein tyrosine kinase activity. These results show that the regulation of gene expression by IFN-alpha include not only the classical IFN-alpha-stimulated genes but also the coordinated regulation of two PKC-epsilon-related transcripts that appeared to be highly relevant to the biological actions of IFN-alpha.