Expression of the cysteinyl leukotriene 1 receptor in normal human lung and peripheral blood leukocytes
Expression of the cysteinyl leukotriene 1 receptor in normal human lung and peripheral blood leukocytes
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DOI：
10.1164/ajrccm.163.1.2003101
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发表时间：
2001-01-01
影响因子：
24.7
通讯作者：
Evans, JF
中科院分区：
文献类型：
--
作者：
Figueroa, DJ;Breyer, RM;Evans, JF
半胱氨酰白三烯（CysLT）是人类哮喘的重要介质。药理学和临床研究表明，CysLT 通过激活假定的 7 次跨膜结构域、C 蛋白偶联受体 CysLT(1) 受体来发挥大部分支气管收缩和促炎作用。 CysLT(1) 受体的初始分子特征通过原位杂交显示，人肺平滑肌细胞和肺巨噬细胞中存在 CysLT(1) 受体信使 RNA (mRNA)。我们证实了 CysLT(1) 受体的原位杂交分析结果，并首次对人肺中的 CysLT(1) 受体蛋白进行了免疫组织化学表征。肺中 CysLT(1) 受体的鉴定与 CysLT(1) 受体拮抗剂的抗支气管收缩和抗炎作用一致。我们还报告了大多数外周血嗜酸性粒细胞和前粒细胞 CD34(+) 细胞以及单核细胞和 B 淋巴细胞亚群中 CysLT(1) 受体 mRNA 和蛋白的表达。
The cysteinyl leukotrienes (CysLTs) are important mediators of human asthma. Pharmacologic and clinical studies show that the CysLTs exert most of their bronchoconstrictive and proinflammatory effects through activation of a putative, 7-transmembrane domain, C-protein-coupled receptor, the CysLT(1) receptor. The initial molecular characterization of the CysLT(1) receptor showed by in situ hybridization, the presence of CysLT(1) receptor messenger RNA (mRNA) in human lung smooth-muscle cells and lung macrophages. We confirmed the results of these in situ hybridization analyses for the CysLT(1) receptor, and produced the first immunohistochemical characterization of the CysLT(1) receptor protein in human lung. The identification of the CysLT(1) receptor in the lung is consistent with the antibronchoconstrictive and antiinflammatory actions of CysLT(1) receptor antagonists. We also report the expression of CysLT(1) receptor mRNA and protein in most peripheral blood eosinophils and pregranulocytic CD34(+) cells, and in subsets of monocytes and B lymphocytes.