Selenium Nanoparticles-Embedded Chitosan Microspheres and Their Effects Upon Alcohol-Induced Gastric Mucosal Injury in Rats: Rapid Preparation, Oral Delivery, and Gastroprotective Potential of Selenium Nanoparticles
Selenium Nanoparticles-Embedded Chitosan Microspheres and Their Effects Upon Alcohol-Induced Gastric Mucosal Injury in Rats: Rapid Preparation, Oral Delivery, and Gastroprotective Potential of Selenium Nanoparticles
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DOI：
10.2147/ijn.s237089
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发表时间：
2020-01-01
影响因子：
8
通讯作者：
Hong, Zhuan
中科院分区：
文献类型：
--
作者：
Bai, Kaikai;Hong, Bihong;Hong, Zhuan
工作背景：硒（Se）是动物和人类必需的微量元素，而硒缺乏可加速过量饮酒所致急性胃损伤的发生。硒纳米粒作为一种特殊的硒补充剂，具有良好的性质和独特的生物活性，有望在胃保护方面发挥被动作用。据我们所知，SeNPs的胃保护潜力是未知的，并且还需要可用于临床中的预期商业应用的口服稳定的SeNPs的快速制备。结果：本研究采用一种快速、环保、经济的制备方法，即壳聚糖修饰硒纳米颗粒，超滤纯化硒纳米颗粒，喷雾干燥制备硒纳米颗粒微球。将粒径在60 nm左右的硒纳米粒子均匀地负载到CS-微球中，在胃内条件下，硒纳米粒子可以从微球中释放出来。此外，硒纳米颗粒-CM在硒剂量方面比亚硒酸钠更安全，LD_（50）约为亚硒酸钠的8倍，并能有效提高缺硒大鼠的硒潴留。此外，SeNPs-CM预处理可以显着减轻乙醇诱导的胃粘膜损伤，基于组织学评价。这可能部分归因于SeNPs-CM的系统抗氧化活性，反映在脂质过氧化反应的减少、抗氧化酶活性的增强以及减少侵袭性一氧化氮（NO）。结论：SeNPs-CM可以考虑作为口服SeNPs的潜在补硒剂，对乙醇引起的胃粘膜损伤具有显着的胃保护作用。
Background: Selenium (Se) is an indispensable trace element required for animals and human beings, whereas Se-deficiency can accelerate the development of acute gastric injury induced by over-consumption of alcohol. Selenium nanoparticles (SeNPs), as a special Se-supplement with favorable properties and unique bioactivities, are expected to play a passive role in gastroprotection. To the best of our knowledge, the gastroprotective potential of SeNPs is unknown and also, a rapid preparation of orally stable SeNPs available for prospective commercial application in the clinic is needed. Thus, SeNPs-embedded chitosan microspheres (SeNPs-CM) were developed to deliver SeNPs, and their gastroprotective potential was evaluated.Results: Herein, a rapid, eco-friendly and economic preparation process, composed of synthesis of SeNPs decorated by chitosan (CS), purification of CS-SeNPs by ultra-filtration (UF) and spray-drying of the purified CS-SeNPs, was introduced to prepare SeNPs-CM. The uniformly distributed SeNPs with a nanosize range of 60 nm were loaded into CS-microspheres, and they could be released from the microspheres in gastric conditions. In addition, SeNPs-CM were safer than selenite in terms of Se dose, with a LD50 of around 8-fold of that of selenite, and it could efficiently enhance the Se retention in Se-deficient Wistar rats. Furthermore, SeNPs-CM pre-treatment might significantly attenuate the ethanol-induced gastric mucosal damage, based on histological evaluation. It might be partly attributed to the systematic antioxidant activities of SeNPs-CM, reflected by the reduction in lipid peroxidation, the augmentation in antioxidant enzymatic activity as well as decreasing aggressive nitric oxides (NO).Conclusion: SeNPs-CM could be taken into consideration as a prospective Se-supplement for the oral delivery of SeNPs, with prominent gastroprotective effect against ethanol-induced mucosal injury.