NOP56 negatively regulates MyD88-mediated NF-KB signaling in miiuy croaker, Miichthys miiuy
NOP56 negatively regulates MyD88-mediated NF-KB signaling in miiuy croaker, Miichthys miiuy
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NOP56 负向调节米氏黄花鱼中 MyD88 介导的 NF-KB 信号传导
DOI：
10.1016/j.fsi.2021.11.011
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发表时间：
2022
影响因子：
4.7
通讯作者：
Xu Tianjun
中科院分区：
文献类型：
--
作者：
Ren Xiaomeng;Zhang Han;Yan Xiaolong;Sun Yuena;Xu Tianjun
MyD 88是TLR信号通路中的一个重要衔接子，可激活NF-κB信号通路，促进促炎细胞因子的产生。然而，调节MyD 88表达的分子机制，特别是在硬骨鱼类中，在很大程度上仍然未知。在这项研究中，我们发现NOP 56作为MyD 88介导的NF-κB信号通路的负调控因子。NOP 56过表达抑制MyD 88蛋白表达。而siRNA敲除NOP 56则具有相反的效果。此外，我们发现NOSIC结构域在蛋白水平上负责NOP 56对MyD 88表达的抑制作用。因此，我们将NOP 56作为MyD 88介导的NF-κB信号的负调控因子，通过抑制MyD 88的表达，对硬骨鱼类的调控机制提供了新的见解。
MyD88 is a critical adaptor in the TLRs signaling pathway, which can activate NF-κB signaling pathway and promote proinflammatory cytokines production. However, the molecular mechanisms that modulate MyD88 expression, especially in teleost, remain largely unknown. In this study, we showed that NOP56 serve as a negative regulator of the MyD88-mediated NF-κB signaling pathway. NOP56 overexpression inhibited the protein expression of MyD88. Whereas, siRNA knockdown of NOP56 had opposite effect. Furthermore, we found that the NOSIC domain is responsible for the suppressive effect of NOP56 in MyD88 expression at protein level. Therefore, we identified NOP56 as a negative regulator of MyD88-mediated NF-κB signaling by inhibiting MyD88 expression and provided new insight into the regulation mechanism in teleost fish.