Plasma Membrane‐associated Calcium Signaling Modulates Cadmium Transport
Plasma Membrane‐associated Calcium Signaling Modulates Cadmium Transport
复制标题
质膜相关钙信号调节镉转运
DOI：
10.1111/nph.18698
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发表时间：
2022
期刊：
影响因子：
9.4
通讯作者：
Cun Wang
中科院分区：
文献类型：
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作者：
Yanting Zhang;Zhangqing Wang;Yisong Liu;Tianqi Zhang;Jiaming Liu;Zhang You;Panpan Huang;Zhenqian Zhang;Cun Wang
镉（Cd）是一种对植物生长发育有毒的重金属元素，植物已经进化出许多应对镉胁迫的策略。然而，植物如何感知镉胁迫以及调节转运蛋白功能的机制仍非常基础。在此，我们发现镉胁迫在拟南芥根中诱导出明显的钙离子信号。此外，我们通过多种蛋白质相互作用技术鉴定出钙依赖性蛋白激酶CPK21和CPK23与镉转运蛋白NRAMP6相互作用。然后我们证实，在镉胁迫下，cpk21 23双突变体显著增强了cpk23单突变体的敏感表型，而CPK21和CPK23的过表达及持续激活增强了植物对镉胁迫的耐受性。在酵母和植物中进行的多项生化和生理分析表明，CPK21/23主要在丝氨酸489和苏氨酸505位点磷酸化NRAMP6，以抑制NRAMP6的镉转运活性，从而提高植物的镉耐受性。综上所述，我们发现了一种与质膜相关的钙信号，它调节镉耐受性。这些结果为作物耐镉胁迫的分子育种提供了新的见解。
Cadmium (Cd) is a toxic heavy element for plant growth and development, plants have evolved many strategies to cope with Cd stress. However, the mechanisms how plants sense Cd stress and regulate the function of transporters remain very rudimentary. Here, we found that Cd stress induces obvious Ca2+ signals in Arabidopsis roots. Furthermore, we identified the calcium-dependent protein kinases CPK21 and CPK23 that interacted with the Cd transporter NRAMP6 through a variety of protein interaction techniques. Then we confirmed that the cpk21 23 double mutants significantly enhanced the sensitive phenotype of cpk23 single mutant under Cd stress, while the overexpression and continuous activation of CPK21 and CPK23 enhanced plants tolerance to Cd stress. Multiple biochemical and physiological analyses in yeast and plants demonstrated that CPK21/23 phosphorylate NRAMP6 primarily at Ser489 and Thr505 to inhibit the Cd transport activity of NRAMP6, thereby improving the Cd tolerance of plants. Taken together, we found a plasma membrane-associated calcium signaling that modulates Cd tolerance. These results provide new insights into the molecular breeding of crop tolerance to Cd stress.