Brain microRNAs among social and solitary bees
Brain microRNAs among social and solitary bees
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DOI：
10.1098/rsos.200517
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发表时间：
2019-08
影响因子：
3.5
通讯作者：
K. Kapheim;Beryl M. Jones;Eirik Søvik;Eckart Stolle;Robert M. Waterhouse;G. Bloch;Y. Ben-Shahar
中科院分区：
文献类型：
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作者：
K. Kapheim;Beryl M. Jones;Eirik Søvik;Eckart Stolle;Robert M. Waterhouse;G. Bloch;Y. Ben-Shahar
昆虫向社会生活方式的进化转变与基因表达的谱系特异性变化有关，但驱动这些调节变化的关键节点尚不清楚。我们研究了社会组织和谱系特异性 microRNA (miRNA) 之间的关系。对 12 个蜜蜂物种的基因组扫描表明，miRNA 拷贝数大多是保守的，与社会性无关。然而，对六种蜜蜂的小 RNA 进行深度测序发现，检测到的 miRNA 中很大一部分（20-35%）在大脑中具有谱系特异性表达，其中 24-72% 在其他物种中没有同源物。谱系特异性 miRNA 不成比例地靶向谱系特异性基因，并且表达水平低于共享 miRNA。谱系特异性 miRNA 的预测靶标并未富集具有种姓偏向表达的基因或社会物种正选择下的基因。总之，这些结果表明，新的 miRNA 可能与新的基因共同进化，从而有助于蜜蜂谱系特异性的进化模式，但似乎对社会进化没有显着影响。我们的分析还支持这样的假设，即许多新的 miRNA 由于对 mRNA 靶标的有害影响而被选择清除，并表明基因组结构在调节蜜蜂 miRNA 进化方面的影响力不如哺乳动物 miRNA 所显示的那样。
Evolutionary transitions to a social lifestyle in insects are associated with lineage-specific changes in gene expression, but the key nodes that drive these regulatory changes are unknown. We examined the relationship between social organization and lineage-specific microRNAs (miRNAs). Genome scans across 12 bee species showed that miRNA copy-number is mostly conserved and not associated with sociality. However, deep sequencing of small RNAs in six bee species revealed a substantial proportion (20–35%) of detected miRNAs had lineage-specific expression in the brain, 24–72% of which did not have homologues in other species. Lineage-specific miRNAs disproportionately target lineage-specific genes, and have lower expression levels than shared miRNAs. The predicted targets of lineage-specific miRNAs are not enriched for genes with caste-biased expression or genes under positive selection in social species. Together, these results suggest that novel miRNAs may coevolve with novel genes, and thus contribute to lineage-specific patterns of evolution in bees, but do not appear to have significant influence on social evolution. Our analyses also support the hypothesis that many new miRNAs are purged by selection due to deleterious effects on mRNA targets, and suggest genome structure is not as influential in regulating bee miRNA evolution as has been shown for mammalian miRNAs.