NICOTINE PRODUCTION AND GROWTH OF EXCISED TOBACCO ROOT CULTURES

NICOTINE PRODUCTION AND GROWTH OF EXCISED TOBACCO ROOT CULTURES
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DOI:
10.1104/pp.32.5.480
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发表时间:
1957-01-01
期刊:
影响因子:
7.4
通讯作者:
SOLT, ML
SOLT, ML
中科院分区:
生物学1区
文献类型:
--
作者:
SOLT, ML

文献摘要

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以土耳其烟草(Nicotiana tabacum var. Turkish)无性系根茎为材料,对其生长与烟碱产生的相互关系进行了研究。在指数生长阶段,烟碱的产生速率为每毫米长0.191 [mu]g或每毫克干重29.05 [mu]g。当根的生长端和成熟区分开培养时,尼古丁只在根尖处增加。使用适当浓度的吲哚乙酸,只在伸长的根中观察到尼古丁合成。
Excised roots of a clone of Nicotiana tabacum var. Turkish were grown in sterile culture, and a detailed study was made of the interrelations of their growth and nicotine production. During the exponential phase of growth the roots produced nicotine at a constant rate of 0.191 [mu]g per mm length or 29.05 [mu]g per mg dry weight. When the growing tips and mature regions of the root were cultured separately, nicotine increases resulted only in the case of the tips. By the use of indoleacetic acid in proper concentration, nicotine synthesis was observed only in those roots where elongation occurred.