CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage
CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage
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早期复发性流产患者蜕膜组织中CircRNA表达谱
DOI：
10.1016/j.gendis.2019.06.003
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发表时间：
2020-09-01
期刊：
影响因子：
6.8
通讯作者：
Gao, Rufei
中科院分区：
文献类型：
--
作者：
Li, Chunli;Chen, Xuemei;Gao, Rufei
环状rna （circRNAs）是一类新型的内源性非编码rna，在基因表达调控中发挥重要作用。本研究旨在评估环状rna在早期复发性流产（RM）患者个体组织中的潜在作用。我们使用微阵列数据在个体组织中构建了circRNA表达谱。与对照组相比，早期RM组共检测到123个差异表达circrna，其中上调78个，下调45个（P < 0.05）。基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析也揭示了特异性环状rna的富集。构建了经过验证的circrna靶向miRNA- mrna网络，大多数circrna含有miRNA结合位点。该网络涉及3个circrna， 27个microrna和82个mrna。选择Hsa_circRNA_103092- miR-224-PRLR网络进行qRT-PCR验证。这些结果表明circRNAs在早期RM患者的个体组织中异常表达，并在早期RM的发展中发挥潜在作用。它为反复流产的机制提供了新的见解。重庆医科大学版权所有制作和托管由爱思唯尔B.V.
Circular RNAs (circRNAs) are a novel class of endogenous noncoding RNAs that play important roles in gene expression regulation. This study aimed to evaluate the poten-tial role of circRNAs in decidual tissue of patients with early recurrent miscarriage (RM). We constructed circRNA expression profiles in decidual tissue using microarray data. A total of 123 differentially expressed circRNAs, including 78 upregulated and 45 downregulated cir-cRNAs were detected in the early RM group compared with the control group (P < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also revealed the enrichment of specific circRNAs. The verified circRNA-targeted miRNA-mRNA network was constructed, most of the circRNAs harbored miRNA binding sites. The network involved 3 circRNAs, 27 microRNAs and 82 mRNAs. Hsa_circRNA_103092- miR-224-PRLR network was selected to verify by qRT-PCR. These results showed that circRNAs are aberrantly expressed in the decidual tissue in early RM patients and play potential roles in the development of early RM. It gives new insights into the mechanism of recurrent miscarriage. Copyright (C) 2019, Chongqing Medical University. Production and hosting by Elsevier B.V.