Expression of PHLPP2 correlates with clinicopathologic characteristics and prognosis in colorectal cancer
Expression of PHLPP2 correlates with clinicopathologic characteristics and prognosis in colorectal cancer
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PHLPP2的表达与结直肠癌的临床病理特征和预后相关
DOI：
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发表时间：
2019
影响因子：
1.4
通讯作者：
Liu Liu
中科院分区：
文献类型：
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作者：
Wu Shu Hua;Xu Xiao Yang;Sun Chen Bo;Wen Fei Fei;He Shuang;Gao Xiang Qian;Liu Yuan Hang;Liu Liu
富含亮氨酸的重复蛋白磷酸酶2(PHLPP2)属于磷酸激酶家族，已被报道在多种癌症中发挥重要作用。然而，PHLPP2在结直肠癌中的表达及其与临床病理特征的相关性尚未确定。本研究的目的是研究PHLPP2在大肠癌中的表达，探讨其在结直肠癌中的作用。应用免疫组织化学方法检测130例大肠癌组织和正常组织中PHLPP2、PTEN、PI3KCA和PI3KCB的表达。用RT-PCR和Western blotting分别检测32对大肠癌组织和相应正常组织中PHLPP2、PTEN、PI3KCA和PI3KCB的表达。PHLPP2在结直肠癌组织中的表达明显低于正常组织。而PHLPP2基因在结直肠癌组织和正常组织中的表达差异无统计学意义。PTEN在左半结直肠癌中无表达，而在右半结直肠癌中PI3KCA和PI3KCB的表达显著高于左半结直肠癌。大肠癌中PHLPP2与p-Akt1呈负相关。大肠癌组织中p-Akt1在PHLPP2(+)/PTEN(+)中的表达明显低于其他组。PHLPP2的表达与肿瘤的分化、侵袭和淋巴结转移有关。Kaplan-Meier分析和多因素分析显示，PHLPP2与结直肠癌的预后密切相关，更重要的是，它是结直肠癌独立的预后因素。结论：PHLPP2可能在结直肠癌的发生、转移和预后中起重要作用。
PH domain leucine-rich repeat protein phosphatase 2 (PHLPP2) belongs to the phosphokinase family, that has been reported to play an important role in several cancers. However, the expression of PHLPP2 and its correlation with clinicopathologic characteristics in colorectal cancer (CRC) have yet to be determined. The aim of this study is to investigate the expression of PHLPP2 and explore its role in CRC. The expression of PHLPP2, PTEN, PI3KCA, and .PI3KCB in 130 cases of CRC and normal tissues was assessed by immunohistochemistry. In addition, the expression of PHLPP2, PTEN, PI3KCA, and PI3KCB in 32 pairs of CRC tissues and their corresponding normal tissues was determined by RT-PCR and western blotting, respectively. PHLPP2 expression in CRC was significantly lower than that of normal tissues. However, PHLPP2 mRNA shows no significant difference between CRC and normal tissue. .PTEN expression in left colorectal cancer (LCC) was absent, while PI3KCA and PI3KCB in right colorectal cancer (RCC) were significantly higher than those in LCC. PHLPP2 was negatively correlated with p-Akt1 in CRC. The expression of p-Akt1 in PHLPP2 (+)/PTEN (+) in CRC tissues was significantly lower than that in other groups. PHLPP2 expression was correlated with differentiation, invasion, and lymph node metastasis. Kaplan-Meier analysis and multivariate analysis reveal that PHLPP2 is closely related to prognosis; more importantly, it is an independent prognostic factor for CRC. In conclusion, PHLPP2 may play a major role in the development, metastasis, and prognosis of CRC.