Label-free imaging of polymeric nanomedicines using coherent anti-stokes Raman scattering microscopy
Label-free imaging of polymeric nanomedicines using coherent anti-stokes Raman scattering microscopy
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DOI：
10.1002/jrs.3170
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发表时间：
2012-05-01
影响因子：
2.5
通讯作者：
Moger, J.
中科院分区：
文献类型：
--
作者：
Garrett, N. L.;Lalatsa, A.;Moger, J.
已知纳米颗粒药物递送极大地提高药物的功效，并且已经发现了广泛的应用，具有不同的给药方法，包括口服、静脉内、经皮和眼部途径。然而，这些纳米颗粒穿过组织、与组织相互作用和修饰组织的机制，以及这与改善药物性能的关系仍不清楚。这些都是需要回答的关键问题，以优化未来药物的性质，剂量率和频率，并减少潜在的副作用。我们回答这些问题的能力受到以下事实的极大阻碍，即目前没有成像模式可用于在没有造影剂或高度侵入性方法的帮助下直接可视化这些小颗粒以及周围组织的结构和功能。目前的成像方式通过外部标记获得纳米颗粒和/或周围组织的图像对比度。我们提出相干反斯托克斯拉曼散射显微镜作为一种新的工具，成像纳米药物载体对生物组织和细胞的背景。版权所有（c）2012约翰威利父子有限公司
Nanoparticulate drug delivery is known to greatly improve the efficacy of pharmaceuticals and has found a wide range of applications with different administration methods including oral, intravenous, transcutaneous and ocular routes. However, the mechanisms by which these nanoparticles travel through, interact with, and modify tissues and how this relates to the improved drug performance are still unclear. These are critical questions that need to be answered to optimise the properties of future pharmaceuticals, dose rates and frequencies, and reduce potential side effects. Our ability to answer these questions is greatly hindered by the fact that there is currently no imaging modality available to directly visualise such small particles and the structure and function of the surrounding tissue, without the aid of contrast agents or highly invasive methods. Current imaging modalities derive image contrast of the nanoparticles and/or the surrounding tissues by means of external labels. We present coherent antistokes Raman scattering microscopy as a novel tool for imaging nanoparticle drug carriers against a background of biological tissues and cells. Copyright (c) 2012 John Wiley & Sons, Ltd.