Hydrogels containing metallic glass sub-micron wires for regulating skeletal muscle cell behaviour
Hydrogels containing metallic glass sub-micron wires for regulating skeletal muscle cell behaviour
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DOI：
10.1039/c5bm00215j
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发表时间：
2015-01-01
影响因子：
6.6
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
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作者：
Ahadian, Samad;Sadeghian, Ramin Banan;Khademhosseini, Ali
具有可调电学和机械性能的水凝胶在组织工程、生物传感和生物机器人领域具有广泛的生物应用。在这项工作中，采用钯基金属玻璃亚微米线（PdMGSMW）来增强甲基丙烯酰明胶（GelMA）凝胶的电导率和机械强度。混合 GelMA-PdMGSMW 水凝胶的电阻率和刚度值随凝胶中亚微米线的浓度而变化。与原始 GelMA 凝胶相比，混合 GelMA-PdMGSMW 凝胶在调节 C2C12 成肌细胞的粘附和铺展方面更有效。在包含 PdMGSMW 并施加电刺激后，GelMA 水凝胶中 C2C12 肌管的形成、收缩性和代谢活性也有所增加。后一种现象可能是由于混合 GelMA 凝胶的导电性所致。
Hydrogels with tunable electrical and mechanical properties have a wide range of biological applications in tissue engineering, biosensing, and biorobotics. In this work, palladium-based metallic glass sub-micron wires (PdMGSMWs) were employed to enhance the conductivity and mechanical strength of gelatin methacryloyl (GelMA) gels. The values of electrical resistivity and stiffness of hybrid GelMA-PdMGSMW hydrogels were varied by the concentration of the sub-micron wires in the gels. Compared with pristine GelMA gels, hybrid GelMA-PdMGSMW gels were more efficient in regulating adhesion and spreading of C2C12 myoblasts. Formation, contractility, and metabolic activity of C2C12 myotubes in GelMA hydrogels also increased upon inclusion of the PdMGSMWs and applying electrical stimulation. The latter phenomenon is likely because of the electrical conductivity of hybrid GelMA gels.