Green ultrasound-assisted extraction of carotenoids based on the bio-refinery concept using sunflower oil as an alternative solvent
Green ultrasound-assisted extraction of carotenoids based on the bio-refinery concept using sunflower oil as an alternative solvent
复制标题
DOI：
10.1016/j.ultsonch.2012.07.005
复制
发表时间：
2013-01-01
影响因子：
8.4
通讯作者：
Chemat, Farid
中科院分区：
文献类型：
--
作者：
Li, Ying;Fabiano-Tixier, Anne Sylvie;Chemat, Farid
设计了一种绿色、廉价、易用的超声波辅助提取新鲜胡萝卜中类胡萝卜素的方法。在这种绿色超声辅助提取(UAE)中，葵花籽油被用作有机溶剂的替代品：这一过程符合绿色提取和生物精炼的概念。首次将这种原始的UAE的加工过程与传统的以正己烷为溶剂的溶剂萃取(CSE)进行了比较。采用响应面法(RSM)和超高效液相色谱-二极管阵列检测器-质谱仪(UPLC-DAD-MS)对阿联酋的最佳条件进行了优化。结果表明，以向日葵为溶剂的超临界流体萃取法仅在20min内获得了最高的β-胡萝卜素产率(334.75 mg/L)，而以正己烷为溶剂的超临界流体萃取法在60min内获得了类似的产率(321.35 mg/L)。最佳提取条件为：胡萝卜油比2：10，超声波强度22.5W cm(-2)，温度40℃，超声时间20min，β-胡萝卜素的得率最高。(C)2012爱思唯尔B.V.保留所有权利。
A green, inexpensive and easy-to-use method for carotenoids extraction from fresh carrots assisted by ultrasound was designed in this work. Sunflower oil was applied as a substitute to organic solvents in this green ultrasound-assisted extraction (UAE): a process which is in line with green extraction and bio-refinery concepts. The processing procedure of this original UAE was first compared with conventional solvent extraction (CSE) using hexane as solvent. Moreover, the UAE optimal conditions for the subsequent comparison were optimized using response surface methodology (RSM) and ultra performance liquid chromatography - diode array detector - mass spectroscopy (UPLC-DAD-MS). The results showed that the UAE using sunflower as solvent has obtained its highest beta-carotene yield (334.75 mg/l) in 20 min only, while CSE using hexane as solvent obtained a similar yield (321.35 mg/l) in 60 min. The green UAE performed under optimal extraction conditions (carrot to oil ratio of 2:10, ultrasonic intensity of 22.5 W cm(-2), temperature of 40 degrees C and sonication time of 20 min) gave the best yield of beta-carotene. (C) 2012 Elsevier B.V. All rights reserved.