Generation of a NKX2.1-p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)
Generation of a NKX2.1-p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)
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DOI：
10.1016/j.scr.2019.101659
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Olmer, Ruth
中科院分区：
文献类型：
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作者：
Drick, Nora;Sahabian, Anais;Olmer, Ruth
肿瘤蛋白p63 （p63）编码p53家族的一种转录因子，是呼吸基底细胞的标志物。以人诱导多能干细胞（hiscs） （MHHi06-A-2）的NKX2.1敲入报告细胞系为基础，利用TALEN技术建立了NKX2.1/p63双转基因敲入报告细胞系。报告基因能够优化和监测hiPSC向NKX2.1/p63双阳性细胞的分化，以及对单阳性或双阳性细胞的富集。
Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells.