Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase):: The key enzyme in U-insertion/deletion RNA editing
Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase):: The key enzyme in U-insertion/deletion RNA editing
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DOI：
10.1016/s0092-8674(02)00647-5
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发表时间：
2002-03-08
期刊：
影响因子：
64.5
通讯作者：
Simpson, L
中科院分区：
文献类型：
--
作者：
Aphasizhev, R;Sbicego, S;Simpson, L
从螺旋体利什曼原虫线粒体中纯化了一个3'端RNA尿苷基转移酶，并从该物种和布鲁氏锥虫中克隆表达了该基因。该酶对Mg2+存在下的3' u加成有特异性。TUTase在体内至少以两种稳定的构型存在：一种含有类似于500 kDa的TUTase低聚物，另一种含有类似于700 kDa的TUTase复合物。抗tutase抗血清特异性地共沉淀一小部分p45和p50 RNA连接酶以及大约40%的引导RNA。通过RNAi抑制原环布氏体TUTase表达下调RNA编辑，似乎影响寄生虫的生存能力。
A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg2+. TUTase is present in vivo in at least two stable configurations: one contains a similar to500 kDa TUTase oligomer and the other a similar to700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.