Protumor role of estrogen receptor expression in oral squamous cell carcinoma cells
Protumor role of estrogen receptor expression in oral squamous cell carcinoma cells
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DOI：
10.1016/j.oooo.2021.04.006
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发表时间：
2021-10-19
影响因子：
2.9
通讯作者：
Noguchi, Makoto
中科院分区：
文献类型：
--
作者：
Akyu (Takei), Rie;Tomihara, Kei;Noguchi, Makoto
目标。越来越多的证据表明雌激素受体（ER）介导的信号传导在多种癌症类型中的作用，这与性类固醇依赖器官中的信号传导不同。然而，其在口腔鳞状细胞癌（OSCC）中的作用尚不清楚。研究设计。我们通过免疫组织化学方法检测了ER α和ER β在人OSCC组织中的表达，并通过免疫印迹和流式细胞术检测了这两种受体在OSCC细胞系中的表达。为了进一步评估内质网介导的信号对口腔癌进展、增殖、侵袭和化疗敏感性的贡献，我们用内质网激动剂β -雌二醇刺激细胞系。鳞癌组织免疫组化分析显示，鳞癌细胞的细胞质和细胞核中均存在ER β。相比之下，在分析的任何病例中均未检测到ER α。此外，β -雌二醇刺激后，OSCC细胞的增殖和侵袭性显著升高。经β -雌二醇预处理后，化疗药物诱导的癌细胞凋亡明显减弱。内质网介导的信号传导通过促进OSCC细胞的增殖、侵袭和化疗耐药，在口腔癌的进展中起着至关重要的作用，这表明它有潜力开发针对这类癌症的新型靶向治疗方法。
Objective. Accumulating evidence has demonstrated the protumor role of estrogen receptor (ER)-mediated signaling in multiple cancer types, which is distinct from this signaling in sex steroid-dependent organs. However, its role in oral squamous cell carcinoma (OSCC) remains unclear.Study Design. We assessed the expression of ER alpha and ER beta in human OSCC tissues by immunohistochemistry and evaluated the expression of both receptors in OSCC cell lines by immunoblotting and flow cytometry. To further assess the contribution of ER-mediated signals to oral cancer progression, proliferation, invasion, and chemosensitivity, cell lines were stimulated with the ER agonist beta-estradiol.Results. lmmunohistochemical analysis of OSCC tissues showed that ER beta was present in the cytoplasm and nuclei of OSCC cells. In contrast, ER alpha was not detected in any of the cases analyzed. Additionally, the proliferation and invasiveness of OSCC cells were significantly elevated following stimulation with beta-estradiol. Chemotherapeutic agent-induced apoptosis of cancer cells was attenuated by pretreatment with beta-estradiol.Conclusions. ER-mediated signaling plays a crucial role in oral cancer progression by facilitating the proliferation, invasion, and chemoresistance of OSCC cells, indicating its potential for developing novel targeted therapies for this type of cancer.